Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8245820	M	M	NA	Agree	0.08	17.83	0.00	61.62	22.63	81.35	NONE	POLYA	0.900709	0.055451	0.043197	0.000643	73.31	1.63	24.83	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8245821	M	M	NA	Agree	0.09	18.85	0.00	61.75	21.96	72.75	NONE	POLYA	0.901404	0.055919	0.042027	0.00065	78.00	1.60	20.18	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8245822	M	M	NA	Agree	0.02	9.17	0.00	33.85	12.44	63.79	NONE	POLYA	0.91524	0.042929	0.040552	0.001279	77.72	1.90	20.19	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8245823	M	M	NA	Agree	0.07	15.81	0.00	56.84	21.70	66.40	NONE	POLYA	0.887851	0.067854	0.043215	0.00108	80.00	1.61	18.14	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8245824	M	M	NA	Agree	0.02	13.91	0.00	59.79	19.12	65.64	NONE	POLYA	0.906232	0.054094	0.038893	0.00078	77.38	1.52	20.92	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8245825	M	M	NA	Agree	0.02	12.81	0.00	59.26	20.73	66.56	NONE	POLYA	0.894726	0.063077	0.041094	0.001103	79.26	1.52	19.03	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8245826	M	M	NA	Agree	0.04	14.89	0.00	55.24	18.32	65.89	NONE	POLYA	0.893342	0.065154	0.039993	0.001511	80.85	1.61	17.35	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8245827	M	M	NA	Agree	0.02	12.49	0.00	54.30	18.72	58.06	NONE	POLYA	0.906781	0.053022	0.039035	0.001162	80.27	1.52	18.02	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does