Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-11-04) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8229918	not_specified	M	NA	Conflict	0.31	1.96	0.00	13.89	10.14	71.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.900971	0.043394	0.041202	0.014434	93.04	2.96	3.87	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8229919	not_specified	F	NA	Conflict	18.27	0.03	0.00	0.15	0.10	0.93	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89776	0.041789	0.040433	0.020018	91.85	3.46	4.54	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8229920	not_specified	M	NA	Conflict	0.17	2.13	0.00	11.84	10.77	63.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.907861	0.041074	0.039339	0.011726	92.47	2.58	4.82	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8229921	not_specified	F	NA	Conflict	15.08	0.03	0.00	0.28	0.08	0.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.907605	0.039591	0.039685	0.013119	92.44	2.71	4.72	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8229922	not_specified	F	NA	Conflict	30.31	0.03	0.00	0.38	0.12	1.53	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.894367	0.046106	0.039597	0.01993	91.94	3.35	4.56	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8229923	not_specified	M	NA	Conflict	0.27	2.94	0.00	12.76	14.15	61.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.900447	0.05018	0.038422	0.01095	92.68	2.40	4.79	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8229924	not_specified	M	NA	Conflict	0.14	2.49	0.00	8.68	11.39	55.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.904735	0.04342	0.038673	0.013171	92.02	2.73	5.11	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8229925	not_specified	F	NA	Conflict	19.66	0.06	0.00	0.13	0.08	0.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893014	0.046293	0.039414	0.021278	91.96	3.47	4.43	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8229926	not_specified	F	NA	Conflict	21.42	0.00	0.00	0.10	0.12	0.64	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.905271	0.04424	0.039535	0.010955	92.68	2.48	4.70	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8229927	not_specified	F	NA	Conflict	25.84	0.08	0.00	0.12	0.15	0.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.908635	0.042754	0.040429	0.008182	88.29	2.22	9.37	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8229928	not_specified	M	NA	Conflict	0.19	2.63	0.00	11.33	9.67	63.13	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903503	0.042964	0.041409	0.012124	92.83	2.64	4.40	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8229929	not_specified	F	NA	Conflict	12.95	0.03	0.00	0.08	0.07	1.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889955	0.041997	0.043894	0.024154	91.42	4.02	4.41	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does