Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8178139	F	F	NA	Agree	117.20	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.851086	0.097329	0.047486	0.004099	83.36	3.83	12.54	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178140	F	F	NA	Agree	116.42	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.845813	0.104273	0.045935	0.003979	83.56	3.82	12.35	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178141	F	F	NA	Agree	129.38	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.84388	0.105837	0.046426	0.003857	83.77	3.92	12.05	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178142	F	F	NA	Agree	118.12	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.841728	0.107065	0.046656	0.004551	83.95	3.86	11.93	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178143	F	F	NA	Agree	108.45	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.846974	0.102706	0.047369	0.002951	82.22	3.68	13.81	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178144	F	F	NA	Agree	107.35	0.00	0.00	0.00	0.01	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.856293	0.093691	0.047046	0.002971	83.49	3.73	12.51	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178145	F	F	NA	Agree	154.17	0.00	0.00	0.04	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.84212	0.108791	0.046503	0.002586	82.42	3.39	13.94	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178146	F	F	NA	Agree	113.49	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.846578	0.104173	0.046268	0.002982	82.39	3.56	13.78	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178147	F	F	NA	Agree	108.52	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.856255	0.094828	0.045701	0.003215	83.06	3.70	12.98	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178148	F	F	NA	Agree	117.24	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.853997	0.096874	0.046958	0.002171	84.06	3.77	11.91	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178149	F	F	NA	Agree	104.25	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.85485	0.094463	0.048566	0.002121	82.53	3.52	13.69	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178150	F	F	NA	Agree	109.92	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.853577	0.096794	0.047277	0.002352	83.43	3.55	12.76	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178151	F	F	NA	Agree	106.08	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.855075	0.096209	0.046479	0.002237	83.45	3.66	12.61	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178152	F	F	NA	Agree	116.64	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.835674	0.116544	0.045427	0.002355	81.32	3.62	14.79	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178153	F	F	NA	Agree	124.50	0.00	0.00	0.00	0.02	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.85043	0.099942	0.046994	0.002634	81.94	3.68	14.12	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178154	F	F	NA	Agree	90.85	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.849881	0.101007	0.046859	0.002253	82.56	3.35	13.82	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178155	F	F	NA	Agree	106.46	0.00	0.00	0.00	0.02	0.14	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.856042	0.094891	0.046657	0.00241	83.78	3.48	12.48	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178156	F	F	NA	Agree	133.09	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.843953	0.106214	0.047414	0.002418	81.75	3.53	14.46	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178157	F	F	NA	Agree	151.12	0.00	0.00	0.00	0.00	0.05	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.840117	0.10998	0.047034	0.002869	79.77	3.33	16.63	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178158	F	F	NA	Agree	116.07	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.842793	0.106093	0.048737	0.002378	80.29	3.40	16.04	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178159	F	F	NA	Agree	159.44	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.841469	0.109687	0.046671	0.002173	80.46	3.40	15.88	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178160	F	F	NA	Agree	188.76	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.831113	0.120108	0.045867	0.002912	79.35	3.28	17.13	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178161	F	M	NA	Conflict	5.65	3.98	0.00	28.19	10.00	70.37	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.839646	0.112246	0.045758	0.002351	79.71	3.34	16.70	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178162	F	F	NA	Agree	135.58	0.15	0.00	0.98	0.39	3.15	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.844753	0.10737	0.045886	0.001991	77.00	3.08	19.69	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178163	F	F	NA	Agree	134.01	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.843269	0.106835	0.047527	0.00237	83.28	3.55	12.91	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178164	F	F	NA	Agree	87.68	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.84328	0.106492	0.048233	0.001995	80.26	3.25	16.22	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178165	F	F	NA	Agree	88.51	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.858165	0.095424	0.044169	0.002243	78.64	3.19	17.93	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178166	F	F	NA	Agree	90.73	0.13	0.00	0.74	0.30	2.06	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.857725	0.095612	0.044594	0.002068	80.67	3.26	15.82	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8178167	F	F	NA	Agree	128.49	0.00	0.00	0.03	0.06	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.849729	0.101114	0.047155	0.002002	77.09	3.36	19.30	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does