Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8152685	M	M	NA	Agree	0.00	8.51	0.00	67.95	19.97	75.64	NONE	POLYA	0.850125	0.102913	0.045921	0.001041	94.19	1.44	4.16	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152686	M	M	NA	Agree	0.02	9.35	0.00	64.84	20.09	75.01	NONE	POLYA	0.857786	0.095376	0.045379	0.001459	94.21	1.50	4.08	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152687	M	M	NA	Agree	0.00	7.95	0.00	67.83	15.98	75.46	NONE	POLYA	0.863932	0.087383	0.045124	0.003561	94.06	1.67	4.07	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152688	M	M	NA	Agree	0.00	9.09	0.00	64.17	19.57	78.88	NONE	POLYA	0.851569	0.097402	0.045128	0.005901	93.64	1.85	4.31	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152689	M	M	NA	Agree	0.07	10.84	0.00	71.32	20.06	74.92	NONE	POLYA	0.840647	0.110002	0.046315	0.003036	94.04	1.63	4.13	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152690	M	M	NA	Agree	0.00	9.32	0.00	75.03	22.33	80.64	NONE	POLYA	0.867841	0.085624	0.045121	0.001414	94.62	1.45	3.74	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152691	M	M	NA	Agree	0.00	9.23	0.00	75.30	23.21	80.86	NONE	POLYA	0.836337	0.112362	0.048913	0.002387	94.49	1.63	3.64	126	LP2 (2026-09-30)	PASS	NonPolyA_perM 126 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152692	M	M	NA	Agree	0.00	9.70	0.00	73.84	23.80	82.33	NONE	POLYA	0.849289	0.101068	0.047159	0.002484	94.56	1.53	3.70	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152693	M	M	NA	Agree	0.00	8.06	0.00	71.37	21.07	77.02	NONE	POLYA	0.869204	0.085473	0.044255	0.001067	94.63	1.50	3.67	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152694	M	M	NA	Agree	0.00	9.56	0.00	58.39	20.02	86.12	NONE	POLYA	0.877096	0.075685	0.045631	0.001588	94.72	1.62	3.47	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152695	M	M	NA	Agree	0.00	8.26	0.00	67.51	21.39	74.33	NONE	POLYA	0.857585	0.089593	0.046625	0.006196	93.78	1.94	4.08	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152696	M	M	NA	Agree	0.00	9.05	0.00	74.76	17.83	88.88	NONE	POLYA	0.895897	0.058039	0.041419	0.004645	94.12	1.88	3.82	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152697	M	M	NA	Agree	0.00	9.29	0.00	73.21	22.09	75.07	NONE	POLYA	0.862223	0.090366	0.044683	0.002727	94.47	1.60	3.73	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152698	M	M	NA	Agree	0.01	11.97	0.00	77.02	23.72	78.44	NONE	POLYA	0.850186	0.100489	0.047633	0.001692	94.40	1.49	3.92	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8152699	M	M	NA	Agree	0.00	9.08	0.00	68.01	22.63	74.89	NONE	POLYA	0.857227	0.094493	0.046905	0.001375	94.25	1.47	4.07	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does