Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8064708	M	M	NA	Agree	0.00	6.46	0.00	30.94	25.81	61.97	NONE	POLYA	0.831456	0.122677	0.043094	0.002773	92.76	1.45	5.62	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8064709	M	M	NA	Agree	0.08	3.51	0.00	21.30	12.92	49.38	NONE	POLYA	0.876211	0.076546	0.041327	0.005916	93.42	1.68	4.74	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8064710	M	M	NA	Agree	0.08	3.81	0.00	21.57	15.50	61.85	NONE	POLYA	0.873875	0.078427	0.042055	0.005643	92.26	1.70	5.89	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8064711	M	M	NA	Agree	0.08	4.30	0.00	30.29	15.65	60.17	NONE	POLYA	0.857427	0.095487	0.041978	0.005108	93.37	1.71	4.74	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8064712	M	M	NA	Agree	0.03	3.51	0.00	26.40	12.10	49.09	NONE	POLYA	0.864554	0.091968	0.040017	0.003461	93.92	1.51	4.36	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8064713	M	M	NA	Agree	0.00	3.96	0.00	32.51	17.60	53.53	NONE	POLYA	0.85936	0.096701	0.040811	0.003128	91.74	1.48	6.57	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8064714	M	M	NA	Agree	0.03	4.05	0.00	25.93	15.58	55.47	NONE	POLYA	0.865923	0.088173	0.041889	0.004015	93.60	1.53	4.70	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8064715	M	M	NA	Agree	0.05	4.15	0.00	25.21	16.70	55.92	NONE	POLYA	0.86035	0.094644	0.042161	0.002845	93.25	1.42	5.17	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8064716	M	M	NA	Agree	0.00	6.68	0.00	35.60	19.46	62.13	NONE	POLYA	0.83715	0.116536	0.043217	0.003097	92.28	1.47	6.06	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does