Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8058084	M	M	NA	Agree	0.00	10.80	0.00	60.63	21.50	70.20	NONE	POLYA	0.86671	0.097473	0.034772	0.001045	89.70	1.21	8.94	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8058085	M	M	NA	Agree	0.00	7.21	0.00	43.19	11.15	68.87	NONE	POLYA	0.886886	0.077142	0.034959	0.001013	89.00	1.48	9.32	114	LP2 (2026-09-30)	PASS	NonPolyA_perM 114 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8058086	M	M	NA	Agree	0.02	6.33	0.00	60.72	12.46	68.48	NONE	POLYA	0.869472	0.097509	0.032121	0.000898	89.80	1.62	8.38	175	LP2 (2026-09-30)	PASS	NonPolyA_perM 175 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8058087	M	M	NA	Agree	0.01	8.54	0.00	49.66	14.11	76.00	NONE	POLYA	0.865031	0.101549	0.032413	0.001006	91.35	1.65	6.79	126	LP2 (2026-09-30)	PASS	NonPolyA_perM 126 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8058088	M	M	NA	Agree	0.00	5.33	0.00	23.69	9.69	57.83	NONE	POLYA	0.906894	0.05674	0.03242	0.003946	93.44	1.84	4.56	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8058089	M	M	NA	Agree	0.02	7.07	0.00	42.08	13.10	59.63	NONE	POLYA	0.878374	0.086389	0.031684	0.003554	93.15	1.55	5.15	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does