Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8003291	M	M	NA	Agree	0.01	8.26	0.00	54.10	23.74	56.22	NONE	POLYA	0.853429	0.09256	0.046882	0.007129	90.60	2.48	6.70	120	LP2 (2026-09-30)	PASS	NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8003292	M	M	NA	Agree	0.00	10.18	0.00	49.91	18.23	69.72	NONE	POLYA	0.854222	0.088444	0.043515	0.013819	89.94	3.13	6.72	130	LP2 (2026-09-30)	PASS	NonPolyA_perM 130 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8003293	M	M	NA	Agree	0.01	10.34	0.00	50.07	23.30	67.47	NONE	POLYA	0.856938	0.094192	0.043564	0.005306	90.57	2.29	6.92	160	LP2 (2026-09-30)	PASS	NonPolyA_perM 160 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8003294	M	M	NA	Agree	0.00	6.45	0.00	41.01	17.06	74.75	NONE	POLYA	0.868759	0.08084	0.043175	0.007226	90.51	2.59	6.66	153	LP2 (2026-09-30)	PASS	NonPolyA_perM 153 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8003295	M	M	NA	Agree	0.02	6.56	0.00	32.15	19.20	61.32	NONE	POLYA	0.872317	0.080211	0.043022	0.00445	91.91	2.25	5.64	129	LP2 (2026-09-30)	PASS	NonPolyA_perM 129 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8003296	M	M	NA	Agree	0.01	6.82	0.00	40.57	15.74	70.10	NONE	POLYA	0.875094	0.074303	0.044108	0.006495	90.41	2.53	6.82	152	LP2 (2026-09-30)	PASS	NonPolyA_perM 152 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does