Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO). For 1 sample processed before workflow 2.1, ComputedSex is the earlier curated call, kept after review where it differed from the rule above.
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8002357	M	M	NA	Agree	0.00	6.48	0.00	27.46	14.74	45.34	NONE	POLYA	0.863863	0.090108	0.043587	0.002441	87.97	1.49	10.40	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002358	M	M	NA	Agree	0.02	5.91	0.00	31.77	14.77	46.48	NONE	POLYA	0.86149	0.091281	0.043494	0.003735	90.13	1.65	8.08	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002359	M	M	NA	Agree	0.02	7.50	0.00	33.63	16.45	55.19	NONE	POLYA	0.850618	0.103826	0.043721	0.001835	89.48	1.45	8.92	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002360	M	M	NA	Agree	0.00	2.02	0.00	11.11	6.70	41.55	NONE	POLYA	0.920489	0.039671	0.033231	0.00661	88.85	2.15	8.90	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002361	M	M	NA	Agree	0.01	7.88	0.00	31.57	17.08	53.70	NONE	POLYA	0.851002	0.100738	0.044802	0.003459	91.85	1.58	6.42	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002362	M	M	NA	Agree	0.01	7.85	0.00	40.58	15.19	70.12	NONE	POLYA	0.855224	0.098126	0.04464	0.00201	91.19	1.57	7.06	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002363	M	M	NA	Agree	0.00	7.67	0.00	35.76	15.58	52.23	NONE	POLYA	0.845952	0.105506	0.045579	0.002962	90.88	1.53	7.44	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002364	M	M	NA	Agree	0.01	7.47	0.00	34.47	14.41	56.51	NONE	POLYA	0.848116	0.101467	0.045839	0.004578	90.69	1.80	7.32	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002365	M	M	NA	Agree	0.01	6.01	0.00	31.70	14.04	51.13	NONE	POLYA	0.865839	0.086455	0.041974	0.005732	90.23	1.88	7.74	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002366	M	M	NA	Agree	0.00	4.19	0.00	22.01	10.21	42.47	NONE	POLYA	0.890145	0.066252	0.040173	0.003431	90.76	1.75	7.37	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002367	M	M	NA	Agree	0.01	6.99	0.00	35.42	15.01	57.84	NONE	POLYA	0.856429	0.097028	0.042776	0.003767	91.13	1.68	7.05	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002368	M	M	NA	Agree	0.01	6.75	0.00	32.67	16.42	49.15	NONE	POLYA	0.842703	0.107704	0.045045	0.004549	91.99	1.72	6.13	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002369	M	M	NA	Agree	0.00	1.41	0.00	6.27	4.78	34.12	NONE	POLYA	0.912854	0.041989	0.036262	0.008895	91.89	2.72	5.30	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002370	M	M	NA	Agree	0.00	6.35	0.00	34.00	18.25	62.05	NONE	POLYA	0.853565	0.097235	0.045323	0.003877	91.47	1.73	6.64	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002371	M	M	NA	Agree	0.00	5.51	0.00	30.63	16.58	58.37	NONE	POLYA	0.841719	0.106959	0.047442	0.003879	91.66	1.74	6.43	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002372	M	M	NA	Agree	0.00	7.61	0.00	38.67	18.40	56.78	NONE	POLYA	0.838248	0.114202	0.045979	0.001571	92.22	1.47	6.15	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002373	M	M	NA	Agree	0.01	5.73	0.00	30.82	13.39	46.10	NONE	POLYA	0.849064	0.105075	0.042965	0.002896	91.88	1.56	6.42	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002374	M	M	NA	Agree	0.00	5.51	0.00	22.65	11.93	38.77	NONE	POLYA	0.870398	0.084953	0.041929	0.002719	92.36	1.64	5.87	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002375	M	M	NA	Agree	0.00	7.06	0.00	33.49	13.79	47.04	NONE	POLYA	0.853357	0.09871	0.044046	0.003887	91.72	1.68	6.44	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002376	M	M	NA	Agree	0.00	2.84	0.00	13.92	6.50	49.16	NONE	POLYA	0.900275	0.045067	0.043245	0.011413	91.36	2.79	5.72	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002377	M	M	NA	Agree	0.01	4.93	0.00	25.81	11.49	52.45	NONE	POLYA	0.871649	0.080971	0.04161	0.005769	91.80	1.85	6.21	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002378	M	M	NA	Agree	0.03	6.17	0.00	31.54	13.75	48.80	NONE	POLYA	0.843832	0.106535	0.046917	0.002716	91.50	1.60	6.75	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002379	M	M	NA	Agree	0.00	2.58	0.00	14.32	7.49	45.86	NONE	POLYA	0.907896	0.044919	0.037514	0.009672	92.35	2.42	5.12	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002380	M	M	NA	Agree	0.00	2.43	0.00	10.59	5.82	43.76	NONE	POLYA	0.91468	0.041979	0.035294	0.008048	92.88	2.37	4.65	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002381	M	M	NA	Agree	0.00	7.64	0.00	31.03	14.11	48.88	NONE	POLYA	0.845222	0.106485	0.044968	0.003325	92.80	1.67	5.37	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002382	M	M	NA	Agree	0.00	5.85	0.00	29.11	13.17	46.21	NONE	POLYA	0.850188	0.10258	0.042818	0.004414	92.05	1.68	6.13	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002383	M	M	NA	Agree	0.00	6.70	0.00	31.55	13.01	44.08	NONE	POLYA	0.852074	0.098751	0.043519	0.005655	91.38	1.82	6.66	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002384	M	M	NA	Agree	0.00	8.18	0.00	36.68	15.75	51.52	NONE	POLYA	0.844383	0.105976	0.044112	0.00553	91.24	1.76	6.84	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002385	M	M	NA	Agree	0.00	6.96	0.00	35.38	16.03	50.63	NONE	POLYA	0.842356	0.107734	0.045396	0.004514	91.40	1.68	6.77	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002386	M	M	NA	Agree	0.00	2.85	0.00	14.95	8.08	41.82	NONE	POLYA	0.903897	0.05451	0.038499	0.003094	90.81	1.90	7.17	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002387	M	M	NA	Agree	0.00	3.90	0.00	18.81	10.09	61.25	NONE	POLYA	0.902441	0.052386	0.037016	0.008158	89.51	2.07	8.31	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002388	M	M	NA	Agree	0.00	10.53	0.00	46.23	25.32	69.60	NONE	POLYA	0.796084	0.146695	0.04906	0.008161	88.85	1.86	9.12	149	LP2 (2026-09-30)	PASS	NonPolyA_perM 149 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002389	M	M	NA	Agree	0.00	2.97	0.00	12.48	7.18	57.61	NONE	POLYA	0.911112	0.042088	0.038735	0.008064	90.16	2.28	7.43	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002390	M	M	NA	Agree	0.00	9.81	0.00	45.66	20.40	68.58	NONE	POLYA	0.808365	0.137407	0.048723	0.005505	88.46	1.63	9.73	157	LP2 (2026-09-30)	PASS	NonPolyA_perM 157 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002391	M	M	NA	Agree	0.00	3.56	0.00	17.23	7.47	64.42	NONE	POLYA	0.912153	0.045802	0.03426	0.007785	89.10	2.15	8.64	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002392	M	M	NA	Agree	0.00	10.19	0.00	43.93	25.94	65.59	NONE	POLYA	0.798321	0.149459	0.050008	0.002212	90.58	1.33	7.91	167	LP2 (2026-09-30)	PASS	NonPolyA_perM 167 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002393	M	M	NA	Agree	0.00	9.36	0.00	42.82	23.82	67.98	NONE	POLYA	0.801708	0.145055	0.04936	0.003877	89.03	1.45	9.35	157	LP2 (2026-09-30)	PASS	NonPolyA_perM 157 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002394	M	M	NA	Agree	0.01	9.67	0.00	49.94	24.16	72.97	NONE	POLYA	0.80946	0.137693	0.049242	0.003605	90.17	1.45	8.21	159	LP2 (2026-09-30)	PASS	NonPolyA_perM 159 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002395	M	M	NA	Agree	0.00	10.30	0.00	42.91	26.99	64.24	NONE	POLYA	0.801281	0.143876	0.050614	0.004228	90.11	1.48	8.23	160	LP2 (2026-09-30)	PASS	NonPolyA_perM 160 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002396	M	M	NA	Agree	0.01	1.30	0.00	9.69	3.92	48.06	NONE	POLYA	0.921654	0.035523	0.030055	0.012768	91.52	2.72	5.63	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002397	M	M	NA	Agree	0.00	1.52	0.00	5.51	3.18	51.14	NONE	POLYA	0.925053	0.03258	0.031116	0.011251	91.98	2.77	5.15	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002398	M	M	NA	Agree	0.00	12.70	0.00	42.31	28.30	75.85	NONE	POLYA	0.79699	0.149202	0.051844	0.001964	90.04	1.35	8.44	177	LP2 (2026-09-30)	PASS	NonPolyA_perM 177 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002399	M	M	NA	Agree	0.01	11.23	0.00	41.12	28.00	67.73	NONE	POLYA	0.800794	0.14664	0.050272	0.002293	89.72	1.37	8.74	164	LP2 (2026-09-30)	PASS	NonPolyA_perM 164 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002400	M	M	NA	Agree	0.01	0.57	0.00	2.31	1.51	30.69	NONE	POLYA	0.919054	0.033381	0.024727	0.022839	91.07	3.97	4.89	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002401	M	M	NA	Agree	0.00	11.18	0.00	31.96	26.32	63.82	NONE	POLYA	0.794651	0.152131	0.051528	0.00169	89.46	1.39	8.96	176	LP2 (2026-09-30)	PASS	NonPolyA_perM 176 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002402	M	M	NA	Agree	0.00	9.92	0.00	37.33	23.99	76.02	NONE	POLYA	0.785931	0.158564	0.051204	0.004301	89.54	1.61	8.65	177	LP2 (2026-09-30)	PASS	NonPolyA_perM 177 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002403	M	M	NA	Agree	0.00	10.99	0.00	43.81	28.78	71.66	NONE	POLYA	0.793255	0.154325	0.049642	0.002779	88.67	1.38	9.77	157	LP2 (2026-09-30)	PASS	NonPolyA_perM 157 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002404	M	M	NA	Agree	0.00	12.16	0.00	44.45	26.61	68.73	NONE	POLYA	0.793268	0.153827	0.049408	0.003497	90.76	1.49	7.58	180	LP2 (2026-09-30)	PASS	NonPolyA_perM 180 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002405	M	M	NA	Agree	0.00	5.63	0.00	26.07	14.14	65.83	NONE	POLYA	0.867714	0.083633	0.042546	0.006107	90.96	1.85	7.04	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002406	M	M	NA	Agree	0.00	11.42	0.00	44.55	26.32	67.55	NONE	POLYA	0.803291	0.144236	0.049967	0.002506	90.73	1.39	7.71	173	LP2 (2026-09-30)	PASS	NonPolyA_perM 173 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002407	M	M	NA	Agree	0.04	2.38	0.00	14.14	6.89	54.76	NONE	POLYA	0.909251	0.045802	0.037776	0.007171	92.01	2.28	5.58	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002408	M	M	NA	Agree	0.00	2.90	0.00	13.71	6.44	62.30	NONE	POLYA	0.912191	0.045367	0.034123	0.00832	91.20	2.29	6.39	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002409	M	M	NA	Agree	0.00	0.19	0.00	0.85	0.62	20.51	NONE	POLYA	0.933697	0.030303	0.022344	0.013656	92.35	3.69	3.90	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002410	M	M	NA	Agree	0.00	10.71	0.00	46.40	26.04	71.96	NONE	POLYA	0.791842	0.156101	0.05019	0.001867	91.40	1.35	7.06	187	LP2 (2026-09-30)	PASS	NonPolyA_perM 187 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002411	M	M	NA	Agree	0.00	1.00	0.00	3.47	2.09	34.06	NONE	POLYA	0.936684	0.025641	0.025508	0.012166	92.73	2.87	4.30	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002412	M	M	NA	Agree	0.00	10.06	0.00	43.80	25.55	79.07	NONE	POLYA	0.802193	0.142615	0.05007	0.005122	90.68	1.65	7.49	154	LP2 (2026-09-30)	PASS	NonPolyA_perM 154 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002413	M	M	NA	Agree	0.00	10.53	0.00	49.30	24.08	63.55	NONE	POLYA	0.792281	0.155447	0.048888	0.003384	91.67	1.47	6.67	193	LP2 (2026-09-30)	PASS	NonPolyA_perM 193 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002414	M	M	NA	Agree	0.00	10.03	0.00	47.63	22.60	71.72	NONE	POLYA	0.806186	0.136765	0.050582	0.006467	90.06	1.80	7.94	161	LP2 (2026-09-30)	PASS	NonPolyA_perM 161 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002415	M	M	NA	Agree	0.01	11.46	0.00	45.49	24.82	69.84	NONE	POLYA	0.789064	0.158503	0.048866	0.003567	91.62	1.45	6.74	164	LP2 (2026-09-30)	PASS	NonPolyA_perM 164 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002416	M	M	NA	Agree	0.00	10.54	0.00	43.47	26.31	68.68	NONE	POLYA	0.791252	0.15519	0.04964	0.003918	90.55	1.50	7.77	162	LP2 (2026-09-30)	PASS	NonPolyA_perM 162 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8002417	M	M	NA	Agree	0.00	0.77	0.00	2.63	1.40	32.90	NONE	POLYA	0.929513	0.031107	0.023904	0.015476	92.42	3.34	4.17	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does