Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO). For 1 sample processed before workflow 2.1, ComputedSex is the earlier curated call, kept after review where it differed from the rule above.
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7981264	M	M	NA	Agree	0.01	1.59	0.00	7.78	4.30	22.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.909581	0.039437	0.028619	0.022363	81.93	10.83	6.34	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981265	M	M	NA	Agree	0.01	1.52	0.00	6.64	3.94	20.72	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.916137	0.039869	0.0281	0.015894	84.10	10.09	5.00	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981266	M	M	NA	Agree	0.02	1.11	0.00	8.20	4.43	18.51	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.916257	0.039227	0.029442	0.015073	76.23	10.25	12.80	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981267	M	M	NA	Agree	0.01	1.48	0.00	8.03	5.10	25.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925534	0.039249	0.029502	0.005715	76.13	8.91	14.38	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981268	M	M	NA	Agree	0.01	1.13	0.00	6.36	2.87	24.31	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.916996	0.030901	0.026988	0.025115	81.45	11.80	5.87	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981269	M	M	NA	Agree	0.01	1.36	0.00	5.34	3.84	18.46	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868909	0.029819	0.025588	0.075683	72.61	15.48	11.26	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981270	M	M	NA	Agree	0.00	0.03	0.00	0.09	1.10	0.99	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925184	0.016167	0.02259	0.036059	78.79	15.59	4.81	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981271	M	M	NA	Agree	0.01	0.54	0.00	4.63	1.80	15.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.939656	0.027254	0.023715	0.009374	75.23	12.42	11.77	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981272	M	M	NA	Agree	0.00	1.17	0.00	5.52	3.19	19.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903947	0.032044	0.027097	0.036913	78.71	12.84	7.76	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981273	M	M	NA	Agree	0.00	1.86	0.00	11.11	6.06	38.95	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.915795	0.042009	0.03092	0.011276	82.86	9.08	7.35	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981274	M	M	NA	Agree	0.00	1.41	0.00	7.39	4.08	27.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925004	0.033373	0.028013	0.01361	83.29	10.00	6.02	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981275	M	M	NA	Agree	0.01	1.11	0.00	7.25	3.53	23.97	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.923781	0.034258	0.027458	0.014504	79.91	10.40	9.02	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981276	M	M	NA	Agree	0.01	1.15	0.00	4.67	3.07	16.37	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.928749	0.033159	0.026862	0.01123	81.16	9.78	8.21	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981277	M	M	NA	Agree	0.00	1.34	0.00	4.54	3.25	14.48	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.931833	0.033041	0.026746	0.00838	79.80	10.21	9.21	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981278	M	M	NA	Agree	0.01	0.90	0.00	2.95	2.78	11.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.929778	0.030158	0.026539	0.013525	80.30	11.14	7.77	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981279	M	M	NA	Agree	0.00	0.87	0.00	4.19	3.28	22.52	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.932349	0.028869	0.026106	0.012676	81.99	11.40	5.90	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981280	M	M	NA	Agree	0.04	2.09	0.00	3.51	7.79	39.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.924374	0.038116	0.029925	0.007585	84.54	7.98	6.92	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981281	M	M	NA	Agree	0.01	1.20	0.00	5.93	3.40	18.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.924509	0.029883	0.025615	0.019992	79.84	11.25	8.20	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981282	M	M	NA	Agree	0.00	0.67	0.00	5.06	3.34	20.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.930287	0.032065	0.027096	0.010552	82.16	10.46	6.63	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981283	M	M	NA	Agree	0.00	0.41	0.00	2.55	2.15	13.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.912826	0.021269	0.022085	0.04382	73.38	13.18	12.68	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981284	M	M	NA	Agree	0.01	0.90	0.00	5.13	3.49	18.52	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.933995	0.030951	0.024892	0.010162	82.54	9.99	6.67	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981285	M	M	NA	Agree	0.00	0.39	0.00	2.21	1.76	9.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.939268	0.025422	0.022153	0.013157	79.69	12.92	6.68	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981286	M	M	NA	Agree	0.00	0.91	0.00	4.98	3.34	17.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.935759	0.030977	0.02694	0.006324	78.29	10.42	10.60	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981287	M	M	NA	Agree	0.02	1.59	0.00	8.26	4.80	32.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.927964	0.037462	0.028329	0.006246	78.40	9.11	11.84	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981288	M	M	NA	Agree	0.03	10.01	0.00	44.98	23.21	63.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.887528	0.064689	0.042293	0.00549	89.71	3.46	6.16	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981289	M	M	NA	Agree	0.01	8.66	0.00	45.33	20.71	60.45	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893627	0.06028	0.041215	0.004878	88.47	3.45	7.46	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981290	M	M	NA	Agree	0.05	8.63	0.00	41.90	20.29	63.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89059	0.054145	0.040154	0.015112	88.56	4.42	6.45	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981291	M	M	NA	Agree	0.03	9.45	0.00	45.37	21.11	64.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884436	0.057359	0.041472	0.016733	88.68	4.54	6.11	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981292	M	M	NA	Agree	0.07	9.88	0.00	42.86	21.73	58.63	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886495	0.06331	0.042233	0.007962	88.18	3.71	7.41	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981293	M	M	NA	Agree	0.10	8.65	0.00	44.62	19.97	61.78	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890483	0.062774	0.040566	0.006176	90.00	3.56	5.82	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981294	M	M	NA	Agree	0.08	8.37	0.00	45.23	19.32	67.45	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.867982	0.066192	0.040675	0.02515	86.52	5.46	7.35	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981295	M	M	NA	Agree	0.06	9.11	0.00	43.93	20.13	67.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89083	0.062654	0.040012	0.006504	90.08	3.58	5.68	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981296	M	M	NA	Agree	0.08	8.80	0.00	38.43	17.88	63.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890289	0.061069	0.04072	0.007922	89.49	3.73	6.09	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981297	M	M	NA	Agree	0.07	9.45	0.00	45.76	22.19	60.52	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.875868	0.065789	0.040103	0.01824	88.59	4.68	6.07	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981298	M	M	NA	Agree	0.08	8.81	0.00	40.54	19.04	66.64	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89458	0.059107	0.040317	0.005996	90.22	3.56	5.56	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981299	M	M	NA	Agree	0.05	9.66	0.00	45.44	19.72	64.56	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.892537	0.061135	0.041502	0.004826	91.35	3.44	4.47	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981300	M	M	NA	Agree	0.07	10.53	0.00	52.40	21.28	66.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89288	0.061135	0.040683	0.005302	90.57	3.53	5.41	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981301	M	M	NA	Agree	0.09	8.85	0.00	26.92	21.51	76.89	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.897552	0.05657	0.041953	0.003925	90.03	3.44	5.89	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981302	M	M	NA	Agree	0.05	9.93	0.00	54.21	21.66	66.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.896933	0.05838	0.040949	0.003737	90.62	3.42	5.46	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981303	M	M	NA	Agree	0.07	9.49	0.00	25.90	19.79	82.94	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.896677	0.05593	0.041361	0.006032	91.15	3.64	4.59	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981304	M	M	NA	Agree	0.11	10.29	0.00	44.90	18.89	73.45	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.888714	0.061856	0.041082	0.008348	89.66	3.95	5.71	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981305	M	M	NA	Agree	0.11	10.27	0.00	49.13	20.52	74.36	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.892089	0.06034	0.041147	0.006424	90.08	3.76	5.48	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981306	M	M	NA	Agree	0.56	8.60	0.00	47.79	18.90	66.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886583	0.064597	0.041054	0.007766	89.82	3.83	5.51	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981307	M	M	NA	Agree	0.06	3.42	0.00	11.32	8.61	51.92	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.91393	0.037502	0.03937	0.009198	89.74	4.70	4.87	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981308	M	M	NA	Agree	0.20	7.20	0.00	37.19	16.49	60.86	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891966	0.058268	0.038321	0.011445	90.38	4.25	4.64	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7981309	M	M	NA	Agree	0.04	6.14	0.00	24.43	16.78	67.73	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.897378	0.052556	0.041506	0.008561	88.72	4.13	6.40	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does