Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7932730	M	M	NA	Agree	0.00	2.84	0.00	19.70	5.15	61.73	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.949093	0.02887	0.018134	0.003903	93.83	1.84	4.25	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932731	M	M	NA	Agree	0.00	3.09	0.00	18.28	4.79	57.98	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.948367	0.029052	0.019185	0.003396	93.58	1.80	4.54	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932732	M	M	NA	Agree	0.00	5.14	0.00	29.49	7.50	69.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.935416	0.04158	0.020041	0.002963	92.53	1.68	5.70	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932733	M	M	NA	Agree	0.00	4.79	0.00	33.70	6.98	65.99	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.937647	0.039402	0.019177	0.003774	93.64	1.76	4.52	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932734	M	M	NA	Agree	0.00	5.63	0.00	36.47	8.23	69.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.932045	0.044329	0.020682	0.002944	93.45	1.70	4.75	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932735	M	M	NA	Agree	0.00	5.74	0.00	27.70	8.22	64.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.936565	0.03981	0.019803	0.003822	92.80	1.75	5.34	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932736	M	M	NA	Agree	0.00	4.78	0.00	31.82	7.23	64.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.938792	0.039124	0.018701	0.003382	93.12	1.65	5.13	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932737	M	M	NA	Agree	0.00	3.21	0.00	17.42	5.32	54.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.949835	0.025634	0.017964	0.006567	93.06	2.03	4.82	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932738	M	M	NA	Agree	0.00	4.14	0.00	18.23	6.07	63.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.951019	0.028871	0.017765	0.002345	93.65	1.62	4.64	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932739	M	M	NA	Agree	0.00	4.87	0.00	30.43	6.39	67.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.940048	0.037428	0.018989	0.003535	93.29	1.75	4.86	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932740	M	M	NA	Agree	0.02	3.88	0.00	29.94	7.35	68.49	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.93945	0.037412	0.019555	0.003584	93.65	1.74	4.52	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932741	M	M	NA	Agree	0.00	5.17	0.00	30.92	7.46	59.82	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.934216	0.041185	0.02032	0.004279	93.48	1.85	4.56	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932742	M	M	NA	Agree	0.00	4.53	0.00	27.43	6.93	59.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.934091	0.037082	0.019037	0.00979	93.09	2.33	4.48	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932743	M	M	NA	Agree	0.00	4.14	0.00	22.40	6.21	60.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.94399	0.033478	0.019268	0.003264	93.18	1.73	4.99	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932744	M	M	NA	Agree	0.00	3.74	0.00	23.79	5.65	63.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.938086	0.03435	0.019452	0.008112	92.33	2.22	5.36	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932745	M	M	NA	Agree	0.00	3.94	0.00	24.77	4.95	65.63	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.940003	0.035069	0.019558	0.00537	92.69	1.97	5.24	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does