Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7898416	M	M	NA	Agree	0.00	11.35	0.00	58.33	24.73	103.51	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.837956	0.106937	0.051142	0.003965	79.51	1.70	18.59	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7898417	M	M	NA	Agree	0.00	11.92	0.00	58.26	25.41	125.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.832879	0.11273	0.050471	0.00392	77.24	1.72	20.85	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7898418	M	M	NA	Agree	0.01	7.80	0.00	42.12	26.92	110.99	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.837551	0.109551	0.050679	0.00222	76.33	1.54	21.96	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7898419	M	M	NA	Agree	0.00	12.79	0.00	60.23	26.61	115.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.837436	0.107986	0.050849	0.003729	75.62	1.71	22.48	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7898420	M	M	NA	Agree	0.00	9.22	0.00	51.74	27.42	109.75	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.807294	0.117204	0.050394	0.025108	74.03	3.43	22.36	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7898421	M	M	NA	Agree	0.00	9.46	0.00	50.77	26.71	109.30	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.833331	0.107144	0.049791	0.009734	75.58	2.27	21.98	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7898422	M	M	NA	Agree	0.00	7.86	0.00	55.21	29.55	103.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.835052	0.110218	0.052765	0.001965	77.67	1.51	20.65	131	LP2 (2026-09-30)	PASS	NonPolyA_perM 131 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7898423	M	M	NA	Agree	0.00	9.23	0.00	58.79	29.46	110.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.833411	0.109045	0.053012	0.004533	76.36	1.74	21.72	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7898424	M	M	NA	Agree	0.00	10.69	0.00	59.42	27.22	113.89	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.818462	0.108029	0.051781	0.021729	73.67	3.19	22.95	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7898425	M	M	NA	Agree	0.00	8.51	0.00	51.81	25.29	98.64	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.840656	0.101149	0.051457	0.006737	77.03	1.91	20.90	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7898426	M	M	NA	Agree	0.00	9.56	0.00	51.29	28.15	99.53	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843933	0.097705	0.05177	0.006592	80.35	1.92	17.57	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7898427	M	M	NA	Agree	0.05	8.53	0.00	45.16	27.20	90.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.851208	0.094398	0.050949	0.003446	78.98	1.64	19.22	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does