Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7887515	M	M	NA	Agree	0.01	0.98	0.00	10.05	7.40	30.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.917061	0.040395	0.031604	0.010941	80.12	8.86	10.22	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887516	M	M	NA	Agree	0.01	1.33	0.00	9.76	7.75	34.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.908216	0.04539	0.032486	0.013908	81.04	8.82	9.37	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887517	M	M	NA	Agree	0.00	1.25	0.00	10.73	8.72	35.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.894664	0.044298	0.031686	0.029352	80.68	10.14	8.38	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887518	M	M	NA	Agree	0.02	0.90	0.00	7.44	5.35	27.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.845244	0.036029	0.029493	0.089234	73.61	16.24	9.32	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887519	M	M	NA	Agree	0.01	1.33	0.00	9.73	6.10	39.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893157	0.042541	0.031546	0.032756	78.50	10.51	10.14	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887520	M	M	NA	Agree	0.00	1.30	0.00	11.05	7.76	34.51	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89506	0.045934	0.031448	0.027557	80.80	10.05	8.39	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887521	M	M	NA	Agree	0.00	1.21	0.00	9.81	6.49	33.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864999	0.040339	0.031747	0.062915	76.57	13.32	9.34	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887522	M	M	NA	Agree	0.00	1.10	0.00	10.81	6.96	32.75	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885648	0.042578	0.031582	0.040193	79.44	11.72	8.07	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887523	M	M	NA	Agree	0.00	0.91	0.00	9.65	7.16	35.36	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.897554	0.044281	0.032848	0.025317	79.66	10.06	9.46	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887524	M	M	NA	Agree	0.00	1.19	0.00	11.20	7.24	38.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.911289	0.041682	0.0339	0.013128	81.97	8.99	8.27	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887525	M	M	NA	Agree	0.00	1.20	0.00	10.96	8.00	38.53	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.907134	0.044163	0.032738	0.015965	81.29	9.42	8.57	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887526	M	M	NA	Agree	0.00	0.81	0.00	11.73	7.71	34.13	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.905718	0.042507	0.033137	0.018637	81.20	9.56	8.48	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887527	M	M	NA	Agree	0.00	0.72	0.00	7.37	4.27	29.24	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.924442	0.034102	0.031992	0.009465	81.64	8.99	8.42	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887528	M	M	NA	Agree	0.02	0.67	0.00	7.79	5.42	28.37	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.911685	0.038543	0.030216	0.019556	81.09	9.87	8.17	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887529	M	M	NA	Agree	0.01	0.84	0.00	7.73	4.76	28.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.902514	0.036497	0.030371	0.030618	78.81	10.62	9.76	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887530	M	M	NA	Agree	0.01	0.81	0.00	7.82	5.55	29.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.923026	0.037914	0.030953	0.008107	80.31	8.63	10.14	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887531	M	M	NA	Agree	0.00	1.04	0.00	7.67	5.57	28.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903142	0.036152	0.029143	0.031563	76.99	10.83	11.34	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887532	M	M	NA	Agree	0.00	1.28	0.00	8.78	4.87	29.03	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.898211	0.03784	0.030232	0.033717	77.39	10.94	10.80	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887533	M	M	NA	Agree	0.00	0.98	0.00	9.19	4.91	27.68	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.877096	0.034493	0.028867	0.059544	75.31	12.98	10.88	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887534	M	M	NA	Agree	0.01	1.03	0.00	8.69	4.50	28.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891203	0.036409	0.029327	0.043061	77.11	11.58	10.44	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887535	M	M	NA	Agree	0.01	0.92	0.00	9.10	5.08	25.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.904331	0.034665	0.027984	0.03302	77.28	10.56	11.41	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887536	M	M	NA	Agree	0.00	1.22	0.00	8.98	4.87	27.53	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.895941	0.03917	0.029217	0.035672	78.31	10.73	10.17	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887537	M	M	NA	Agree	0.00	1.01	0.00	8.74	4.52	24.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.748197	0.031412	0.025186	0.195205	62.72	25.55	11.03	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887538	M	M	NA	Agree	0.00	1.86	0.00	12.05	7.49	36.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891957	0.044052	0.031482	0.032509	77.02	10.02	12.08	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887539	M	M	NA	Agree	0.01	8.89	0.00	44.57	20.87	60.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.898188	0.056467	0.039886	0.005458	86.21	3.33	9.83	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887540	M	M	NA	Agree	0.01	9.22	0.00	36.02	19.69	59.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.902562	0.053579	0.038984	0.004876	89.65	3.38	6.36	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887541	M	M	NA	Agree	0.01	7.24	0.00	32.41	18.08	58.07	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.902712	0.049766	0.040892	0.00663	84.94	3.51	10.96	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887542	M	M	NA	Agree	0.01	9.52	0.00	43.35	18.73	53.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884747	0.070014	0.041351	0.003888	85.79	3.17	10.36	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887543	M	M	NA	Agree	0.04	8.72	0.00	46.10	19.53	64.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.881172	0.072605	0.041501	0.004722	85.07	3.22	11.05	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887544	M	M	NA	Agree	0.01	9.57	0.00	44.59	20.72	58.81	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893998	0.059523	0.040998	0.005481	85.96	3.32	10.09	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887545	M	M	NA	Agree	0.01	10.36	0.00	42.28	18.04	57.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885746	0.068537	0.04081	0.004906	85.40	3.42	10.49	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887546	M	M	NA	Agree	0.06	8.27	0.00	42.21	19.65	64.63	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.894833	0.061074	0.039858	0.004236	88.34	3.28	7.81	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887547	M	M	NA	Agree	0.05	10.43	0.00	43.15	21.47	62.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.883682	0.07199	0.039147	0.005181	87.31	3.26	8.82	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887548	M	M	NA	Agree	0.01	9.08	0.00	43.30	20.51	58.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.881782	0.071381	0.039723	0.007114	88.22	3.47	7.69	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887549	M	M	NA	Agree	0.01	10.19	0.00	45.62	23.31	62.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.870065	0.084904	0.040567	0.004463	87.40	3.11	8.89	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887550	M	M	NA	Agree	0.06	9.75	0.00	44.14	20.12	57.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.877022	0.077642	0.039954	0.005382	86.09	3.30	9.97	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887551	M	M	NA	Agree	0.03	9.86	0.00	44.47	20.84	60.92	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.896947	0.056201	0.04053	0.006322	87.29	3.40	8.68	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887552	M	M	NA	Agree	0.00	10.18	0.00	47.46	19.38	59.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.888886	0.065267	0.039313	0.006534	86.91	3.32	9.17	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887553	M	M	NA	Agree	0.01	9.16	0.00	44.26	19.80	59.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.898073	0.056563	0.040087	0.005277	87.57	3.30	8.47	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887554	M	M	NA	Agree	0.01	9.63	0.00	50.10	20.93	59.51	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.895508	0.061278	0.040494	0.002719	89.60	3.04	6.79	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887555	M	M	NA	Agree	0.02	9.66	0.00	46.94	21.29	58.59	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.877031	0.060351	0.039142	0.023476	83.94	4.72	10.76	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887556	M	M	NA	Agree	0.02	8.98	0.00	46.04	18.90	64.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903102	0.054301	0.037765	0.004832	90.35	3.22	5.86	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887557	M	M	NA	Agree	0.00	8.37	0.00	43.43	19.31	58.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890869	0.064434	0.039501	0.005195	88.39	3.46	7.51	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887558	M	M	NA	Agree	0.03	8.02	0.00	39.60	17.67	56.86	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885303	0.067248	0.039417	0.008032	88.13	3.68	7.50	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887559	M	M	NA	Agree	0.04	10.26	0.00	45.81	21.01	58.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889096	0.067479	0.039563	0.003863	88.83	3.26	7.26	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887560	M	M	NA	Agree	0.05	8.79	0.00	40.85	17.58	58.48	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903468	0.053643	0.038717	0.004172	87.51	3.35	8.47	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7887561	M	M	NA	Agree	0.07	9.77	0.00	48.84	19.35	62.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.887894	0.06655	0.040133	0.005423	85.96	3.35	10.02	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does