Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7871241	M	M	NA	Agree	0.00	8.49	0.00	35.48	18.88	64.89	NONE	POLYA	0.815405	0.108152	0.04502	0.031423	89.19	3.83	6.84	131	LP2 (2026-09-30)	PASS	NonPolyA_perM 131 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871242	F	F	NA	Agree	59.96	0.00	0.00	0.00	0.00	0.08	NONE	POLYA	0.869896	0.063603	0.039231	0.027269	90.09	3.63	6.15	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871243	M	M	NA	Agree	0.00	3.67	0.00	16.54	9.05	64.37	NONE	POLYA	0.88854	0.045448	0.034522	0.031489	88.90	4.04	6.94	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871244	F	F	NA	Agree	107.46	0.00	0.00	0.00	0.04	0.09	NONE	POLYA	0.848969	0.089175	0.043621	0.018235	89.28	2.79	7.79	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871245	M	M	NA	Agree	0.02	2.96	0.00	10.11	7.70	54.15	NONE	POLYA	0.897216	0.035279	0.031491	0.036015	89.23	4.67	5.98	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871246	F	F	NA	Agree	24.47	0.00	0.00	0.00	0.00	0.07	NONE	POLYA	0.889463	0.042376	0.034197	0.033964	88.59	4.31	6.99	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871247	M	M	NA	Agree	0.00	4.92	0.00	21.53	13.75	70.18	NONE	POLYA	0.868666	0.064929	0.040112	0.026293	88.71	3.50	7.67	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871248	F	Undetermined	NA	Conflict	17.68	2.57	0.00	12.58	8.01	31.57	NONE	POLYA	0.875942	0.061403	0.037674	0.02498	89.13	3.38	7.37	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871249	M	F	NA	Conflict	25.02	0.21	0.00	0.75	0.62	7.92	NONE	POLYA	0.891842	0.042182	0.033134	0.032842	88.64	4.21	7.04	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871250	F	F	NA	Agree	60.47	0.09	0.00	0.35	0.23	4.66	NONE	POLYA	0.868108	0.066324	0.039469	0.0261	89.83	3.56	6.49	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871251	M	Undetermined	NA	Conflict	38.13	2.04	0.00	10.30	6.18	30.99	NONE	POLYA	0.864273	0.071691	0.040872	0.023164	89.16	3.25	7.46	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871252	F	F	NA	Agree	25.33	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.904746	0.041086	0.03245	0.021718	90.14	3.33	6.42	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871253	M	M	NA	Agree	0.01	6.15	0.00	25.19	15.84	70.19	NONE	POLYA	0.858316	0.073605	0.041323	0.026756	89.42	3.49	6.95	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871254	F	F	NA	Agree	73.68	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.829773	0.095734	0.043456	0.031036	88.31	3.76	7.79	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871255	M	M	NA	Agree	0.01	1.71	0.00	5.67	5.11	50.79	NONE	POLYA	0.909926	0.034315	0.030233	0.025526	88.49	3.64	7.76	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871256	F	F	NA	Agree	17.36	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.912987	0.037609	0.030805	0.018598	88.98	3.21	7.71	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871257	F	F	NA	Agree	40.50	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.880404	0.055377	0.036281	0.027937	88.47	3.65	7.76	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871258	M	M	NA	Agree	0.00	3.99	0.00	15.05	10.01	55.93	NONE	POLYA	0.880169	0.056772	0.036547	0.026512	86.83	3.60	9.45	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871259	F	F	NA	Agree	14.51	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.905882	0.034992	0.028932	0.030195	90.43	4.18	5.30	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871260	M	M	NA	Agree	0.00	3.48	0.00	12.67	8.39	60.65	NONE	POLYA	0.890282	0.053722	0.034164	0.021833	88.67	3.21	8.02	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871261	F	F	NA	Agree	74.50	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.856601	0.077652	0.039887	0.02586	87.57	3.33	8.95	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871262	M	M	NA	Agree	0.01	1.26	0.00	6.28	3.98	49.63	NONE	POLYA	0.908438	0.035156	0.027815	0.028591	90.04	4.15	5.71	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871263	F	F	NA	Agree	66.65	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.858866	0.074425	0.040033	0.026676	87.75	3.50	8.62	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871264	M	M	NA	Agree	0.00	5.49	0.00	25.25	12.86	44.51	NONE	POLYA	0.861899	0.082078	0.040234	0.015789	88.84	2.61	8.42	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871265	F	F	NA	Agree	54.28	0.00	0.00	0.00	0.04	0.00	NONE	POLYA	0.882665	0.056545	0.038741	0.022049	87.43	3.06	9.39	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871266	M	Undetermined	NA	Conflict	13.35	2.31	0.00	10.20	6.30	27.25	NONE	POLYA	0.89054	0.049008	0.036345	0.024108	87.70	3.31	8.88	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871267	F	F	NA	Agree	47.73	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.897279	0.05293	0.036889	0.012902	88.66	2.34	8.88	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871268	M	M	NA	Agree	0.01	4.83	0.00	19.17	12.30	40.98	NONE	POLYA	0.882346	0.057439	0.038175	0.02204	89.73	3.25	6.90	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871269	F	F	NA	Agree	47.99	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.889835	0.052927	0.037647	0.019591	90.53	2.91	6.44	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871270	M	M	NA	Agree	0.03	6.39	0.00	22.97	11.81	50.47	NONE	POLYA	0.880267	0.071948	0.040972	0.006813	94.74	2.04	3.11	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871271	F	F	NA	Agree	105.15	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.871907	0.080323	0.04388	0.00389	95.16	1.78	2.93	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871272	M	M	NA	Agree	0.00	4.12	0.00	16.01	8.64	41.12	NONE	POLYA	0.881182	0.049672	0.038737	0.030409	88.91	3.92	7.06	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871273	F	F	NA	Agree	71.45	0.00	0.00	0.00	0.00	0.18	NONE	POLYA	0.863989	0.067437	0.040568	0.028006	89.35	3.75	6.77	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871274	M	M	NA	Agree	0.00	5.00	0.00	22.39	11.37	40.73	NONE	POLYA	0.864436	0.073385	0.040303	0.021877	90.07	3.09	6.72	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871275	F	F	NA	Agree	21.50	0.00	0.00	0.00	0.00	0.07	NONE	POLYA	0.904926	0.036357	0.034016	0.024702	89.76	3.63	6.51	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871276	M	M	NA	Agree	0.00	3.53	0.00	15.88	8.91	42.27	NONE	POLYA	0.884223	0.054489	0.037399	0.023889	89.86	3.38	6.65	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871277	F	F	NA	Agree	75.59	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.836217	0.096168	0.043675	0.02394	88.94	3.27	7.64	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871278	M	M	NA	Agree	0.01	3.84	0.00	19.60	10.67	43.05	NONE	POLYA	0.875624	0.064298	0.039009	0.021068	89.02	2.96	7.89	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871279	F	F	NA	Agree	111.40	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.851363	0.089461	0.042149	0.017027	88.80	2.65	8.42	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871280	M	M	NA	Agree	0.00	4.13	0.00	20.78	9.60	41.06	NONE	POLYA	0.882234	0.058394	0.03771	0.021662	90.45	3.17	6.26	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871281	F	F	NA	Agree	40.78	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.881076	0.054902	0.040181	0.023841	89.22	3.39	7.26	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871282	M	M	NA	Agree	0.02	4.57	0.00	23.27	12.03	43.83	NONE	POLYA	0.858398	0.077763	0.041732	0.022107	88.29	3.16	8.42	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871283	F	F	NA	Agree	95.27	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.854547	0.080411	0.04073	0.024311	88.43	3.19	8.24	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871284	M	M	NA	Agree	0.00	4.13	0.00	19.03	10.92	42.06	NONE	POLYA	0.876642	0.064454	0.037385	0.02152	89.43	3.10	7.36	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871285	F	F	NA	Agree	75.61	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.85422	0.071553	0.040063	0.034164	88.23	4.07	7.57	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871286	M	M	NA	Agree	0.00	4.68	0.00	23.28	11.36	39.93	NONE	POLYA	0.859948	0.078698	0.039751	0.021604	90.52	3.05	6.30	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7871287	F	F	NA	Agree	83.29	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.856547	0.077808	0.040778	0.024867	90.24	3.51	6.10	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does