Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7866594	M	M	NA	Agree	0.01	5.03	0.00	34.48	6.25	44.54	NONE	POLYA	0.867326	0.055285	0.033595	0.043795	91.80	5.61	2.49	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866595	M	M	NA	Agree	0.02	5.51	0.00	35.94	5.17	45.54	NONE	POLYA	0.866439	0.041607	0.030077	0.061877	90.52	7.10	2.29	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866596	M	M	NA	Agree	0.02	5.06	0.00	36.07	5.62	39.54	NONE	POLYA	0.865526	0.048065	0.032148	0.054262	91.43	6.35	2.13	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866597	M	M	NA	Agree	0.00	5.71	0.00	40.26	6.00	45.29	NONE	POLYA	0.867807	0.046075	0.031944	0.054174	91.16	6.54	2.20	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866598	M	M	NA	Agree	0.04	5.35	0.00	37.72	5.68	41.47	NONE	POLYA	0.868391	0.048079	0.032892	0.050638	91.43	6.22	2.25	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866599	M	M	NA	Agree	0.03	5.13	0.00	38.80	6.02	43.74	NONE	POLYA	0.869248	0.047122	0.033058	0.050571	91.47	6.07	2.37	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866600	M	M	NA	Agree	0.02	6.63	0.00	40.10	6.09	48.07	NONE	POLYA	0.881418	0.049378	0.034239	0.034965	92.94	4.75	2.20	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866601	M	M	NA	Agree	0.02	5.15	0.00	35.56	5.75	45.30	NONE	POLYA	0.882624	0.042051	0.033161	0.042164	92.33	5.35	2.21	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866602	M	M	NA	Agree	0.02	5.65	0.00	38.12	5.53	45.34	NONE	POLYA	0.874683	0.048497	0.033138	0.043682	91.81	5.74	2.35	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866603	M	M	NA	Agree	0.00	5.15	0.00	35.87	5.53	43.69	NONE	POLYA	0.873496	0.044814	0.031674	0.050016	91.69	5.97	2.24	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866604	M	M	NA	Agree	0.01	2.51	0.00	18.23	2.62	22.43	NONE	POLYA	0.87174	0.054325	0.034869	0.039066	92.52	4.91	2.46	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866605	M	M	NA	Agree	0.01	5.32	0.00	36.41	5.87	44.87	NONE	POLYA	0.876218	0.045536	0.033254	0.044992	92.21	5.46	2.23	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866606	M	M	NA	Agree	0.01	5.16	0.00	37.43	6.63	46.25	NONE	POLYA	0.883717	0.050191	0.035009	0.031084	93.42	4.37	2.11	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866607	M	M	NA	Agree	0.03	4.60	0.00	35.45	5.29	39.44	NONE	POLYA	0.885302	0.043657	0.033129	0.037911	92.90	4.74	2.27	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866608	M	M	NA	Agree	0.01	6.14	0.00	34.09	6.55	48.41	NONE	POLYA	0.876076	0.05362	0.034722	0.035582	92.92	4.71	2.25	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866609	M	M	NA	Agree	0.00	5.41	0.00	35.54	6.33	42.17	NONE	POLYA	0.888078	0.04234	0.032575	0.037008	92.57	4.87	2.46	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866610	M	M	NA	Agree	0.01	5.00	0.00	36.50	5.71	39.61	NONE	POLYA	0.880131	0.048121	0.034537	0.03721	92.56	4.87	2.45	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7866611	M	M	NA	Agree	0.02	5.36	0.00	36.27	5.46	40.14	NONE	POLYA	0.881604	0.051313	0.036142	0.030941	93.52	4.32	2.04	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does