Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7863755	M	M	NA	Agree	0.02	5.92	0.00	21.74	8.79	62.73	NONE	POLYA	0.925484	0.041335	0.030261	0.00292	94.76	2.62	2.46	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863756	M	M	NA	Agree	0.01	7.44	0.00	26.41	10.74	81.07	NONE	POLYA	0.916188	0.048808	0.032843	0.002161	95.78	2.10	1.90	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863757	M	M	NA	Agree	0.01	7.41	0.00	31.93	12.76	70.98	NONE	POLYA	0.902658	0.05852	0.031116	0.007706	94.98	2.24	2.63	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863758	M	M	NA	Agree	0.03	7.55	0.00	20.32	9.64	73.58	NONE	POLYA	0.927493	0.04237	0.027857	0.00228	95.08	2.70	2.05	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863759	M	M	NA	Agree	0.06	4.86	0.00	23.22	10.06	76.21	NONE	POLYA	0.921205	0.04363	0.032545	0.00262	95.28	2.39	2.16	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863760	M	M	NA	Agree	0.00	8.72	0.00	26.43	10.67	81.70	NONE	POLYA	0.910526	0.054999	0.032721	0.001754	95.80	2.03	2.01	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863761	M	M	NA	Agree	0.05	6.61	0.00	31.57	13.80	56.99	NONE	POLYA	0.90904	0.054565	0.033384	0.003011	95.68	1.68	2.51	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863762	M	M	NA	Agree	0.05	11.83	0.00	31.76	11.41	77.30	NONE	POLYA	0.910766	0.054409	0.032015	0.00281	94.81	2.43	2.57	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863763	M	M	NA	Agree	0.00	5.86	0.00	29.49	10.91	51.41	NONE	POLYA	0.901572	0.059114	0.034391	0.004922	94.81	2.18	2.88	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863764	M	M	NA	Agree	0.00	12.27	0.00	34.12	14.15	106.95	NONE	POLYA	0.906442	0.05752	0.031699	0.004339	95.04	2.08	2.65	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863765	M	M	NA	Agree	0.00	10.72	0.00	31.46	14.85	99.90	NONE	POLYA	0.916093	0.048876	0.033438	0.001593	95.84	1.90	2.06	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863766	M	M	NA	Agree	0.00	9.66	0.00	30.89	13.75	83.20	NONE	POLYA	0.907082	0.056087	0.030566	0.006265	95.15	2.12	2.58	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863767	M	M	NA	Agree	0.02	5.83	0.00	21.91	9.34	74.99	NONE	POLYA	0.921683	0.043974	0.032421	0.001922	94.72	2.68	2.43	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863768	M	M	NA	Agree	0.00	7.84	0.00	24.78	10.33	95.39	NONE	POLYA	0.911322	0.051228	0.035615	0.001834	95.49	2.27	2.07	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863769	M	M	NA	Agree	0.00	14.11	0.00	37.37	14.34	83.36	NONE	POLYA	0.921117	0.044056	0.033463	0.001364	95.47	1.67	2.74	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863770	M	M	NA	Agree	0.01	8.53	0.00	24.81	13.27	90.24	NONE	POLYA	0.914321	0.048623	0.034108	0.002948	94.88	2.35	2.56	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863771	M	M	NA	Agree	0.00	10.31	0.00	25.01	11.14	107.74	NONE	POLYA	0.928561	0.033119	0.034729	0.003592	95.31	2.46	2.06	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863772	M	M	NA	Agree	0.00	16.94	0.00	38.09	15.37	117.54	NONE	POLYA	0.914642	0.044924	0.038249	0.002184	95.35	2.08	2.44	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863773	M	M	NA	Agree	0.00	8.04	0.00	26.73	12.57	52.80	NONE	POLYA	0.912351	0.049739	0.035222	0.002689	95.28	1.91	2.69	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863774	M	M	NA	Agree	0.00	7.00	0.00	24.00	12.72	55.58	NONE	POLYA	0.917303	0.04566	0.033818	0.00322	95.27	1.94	2.64	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863775	M	M	NA	Agree	0.00	6.48	0.00	30.66	12.26	60.85	NONE	POLYA	0.909149	0.05048	0.035015	0.005355	94.21	2.18	3.48	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863776	M	M	NA	Agree	0.00	11.69	0.00	29.80	16.33	96.76	NONE	POLYA	0.909749	0.05574	0.032082	0.002429	95.61	1.77	2.44	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863777	M	M	NA	Agree	0.00	9.33	0.00	23.56	13.80	91.07	NONE	POLYA	0.912875	0.051018	0.03459	0.001517	95.83	1.80	2.21	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863778	M	M	NA	Agree	0.00	10.48	0.00	36.88	15.42	70.63	NONE	POLYA	0.911226	0.052735	0.034803	0.001236	94.91	1.50	3.47	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863779	M	M	NA	Agree	0.00	4.83	0.00	24.80	10.69	44.70	NONE	POLYA	0.928189	0.037493	0.029005	0.005313	95.10	1.84	2.91	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863780	M	M	NA	Agree	0.00	4.24	0.00	21.47	8.35	36.21	NONE	POLYA	0.923933	0.041455	0.029003	0.005609	93.92	2.77	3.09	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863781	M	M	NA	Agree	0.00	4.73	0.00	29.26	8.37	42.31	NONE	POLYA	0.919335	0.044745	0.029031	0.006889	94.25	2.29	3.31	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863782	M	M	NA	Agree	0.00	3.98	0.00	21.14	8.55	45.41	NONE	POLYA	0.930844	0.033477	0.029201	0.006478	94.20	2.23	3.40	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863783	M	M	NA	Agree	0.00	5.69	0.00	28.65	11.89	46.16	NONE	POLYA	0.914052	0.051518	0.029935	0.004496	94.98	1.97	2.90	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863784	M	M	NA	Agree	0.00	4.09	0.00	26.64	7.32	37.58	NONE	POLYA	0.918394	0.047303	0.03206	0.002243	94.59	3.01	2.20	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863785	M	M	NA	Agree	0.00	7.82	0.00	26.16	11.01	47.25	NONE	POLYA	0.920967	0.043081	0.031073	0.004879	94.12	2.09	3.63	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863786	M	M	NA	Agree	0.00	4.24	0.00	14.67	10.68	50.27	NONE	POLYA	0.927696	0.038904	0.029422	0.003978	94.08	2.04	3.73	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863787	M	M	NA	Agree	0.01	4.19	0.00	16.57	8.12	29.88	NONE	POLYA	0.91472	0.048208	0.028129	0.008943	92.13	4.55	3.02	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863788	M	M	NA	Agree	0.01	4.74	0.00	32.36	10.42	51.10	NONE	POLYA	0.925584	0.041275	0.029828	0.003314	95.64	1.83	2.39	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863789	M	M	NA	Agree	0.01	6.98	0.00	39.30	12.26	53.57	NONE	POLYA	0.907245	0.056427	0.032726	0.003602	95.58	1.86	2.41	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863790	M	M	NA	Agree	0.00	5.91	0.00	32.99	11.45	49.81	NONE	POLYA	0.888877	0.072852	0.03369	0.00458	94.98	2.40	2.43	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863791	M	M	NA	Agree	0.00	3.84	0.00	20.67	6.77	38.78	NONE	POLYA	0.917736	0.043313	0.029231	0.00972	92.83	3.47	3.51	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863792	M	M	NA	Agree	0.00	4.94	0.00	26.31	9.40	46.05	NONE	POLYA	0.916574	0.051136	0.027879	0.004411	94.76	2.25	2.82	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863793	M	M	NA	Agree	0.00	4.46	0.00	22.87	6.04	42.59	NONE	POLYA	0.935762	0.029402	0.029622	0.005214	94.78	2.78	2.27	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863794	M	M	NA	Agree	0.01	4.37	0.00	22.22	6.99	36.16	NONE	POLYA	0.928798	0.040311	0.02601	0.00488	93.86	3.27	2.66	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863795	M	M	NA	Agree	0.00	5.20	0.00	23.34	9.00	43.38	NONE	POLYA	0.924166	0.041876	0.030933	0.003025	95.45	2.19	2.22	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863796	M	M	NA	Agree	0.00	4.38	0.00	21.38	7.30	37.29	NONE	POLYA	0.933859	0.034168	0.027525	0.004447	94.65	2.31	2.84	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863797	M	M	NA	Agree	0.00	6.61	0.00	28.67	14.15	49.18	NONE	POLYA	0.893276	0.068159	0.033765	0.0048	94.66	2.08	3.07	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863798	M	M	NA	Agree	0.00	5.92	0.00	29.22	11.85	44.56	NONE	POLYA	0.91317	0.051811	0.032622	0.002396	95.33	2.15	2.37	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863799	M	M	NA	Agree	0.00	6.61	0.00	32.68	10.37	47.59	NONE	POLYA	0.905951	0.054447	0.036151	0.003451	95.16	1.95	2.74	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863800	M	M	NA	Agree	0.00	5.55	0.00	31.34	11.41	51.47	NONE	POLYA	0.9036	0.061817	0.031075	0.003509	94.82	2.19	2.81	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863801	M	M	NA	Agree	0.00	6.44	0.00	31.99	12.00	52.15	NONE	POLYA	0.906787	0.058496	0.031884	0.002833	95.54	1.63	2.68	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7863802	M	M	NA	Agree	0.00	5.50	0.00	32.68	9.71	51.03	NONE	POLYA	0.916033	0.050142	0.030814	0.003011	94.95	1.93	2.94	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does