Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7843130	M	M	NA	Agree	0.06	11.45	0.00	39.27	14.31	82.72	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.895888	0.037425	0.042314	0.024374	78.49	5.59	15.50	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7843131	M	M	NA	Agree	0.04	12.16	0.00	45.01	14.47	79.30	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.905889	0.038759	0.042713	0.01264	87.86	5.14	6.56	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7843132	M	M	NA	Agree	0.10	11.45	0.00	37.74	12.76	72.91	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.91472	0.035982	0.039876	0.009422	77.76	4.56	17.26	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7843133	M	M	NA	Agree	0.08	12.92	0.00	48.62	14.27	84.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.906073	0.040418	0.044179	0.00933	83.33	4.34	11.94	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7843134	M	M	NA	Agree	0.11	13.47	0.00	49.30	15.73	80.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.908106	0.041456	0.044303	0.006135	84.46	4.02	11.07	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7843135	M	M	NA	Agree	0.03	13.16	0.00	49.13	14.30	82.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.904159	0.043481	0.044883	0.007477	88.53	4.54	6.32	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7843136	M	M	NA	Agree	0.05	9.55	0.00	40.84	15.38	51.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.86509	0.047715	0.039451	0.047743	75.24	5.73	18.79	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7843137	M	M	NA	Agree	0.08	12.27	0.00	48.64	18.19	56.43	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864961	0.056424	0.043776	0.034839	82.21	5.23	12.02	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7843138	M	M	NA	Agree	0.07	11.63	0.00	42.97	19.22	50.34	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893559	0.055947	0.042296	0.008198	87.03	2.84	9.84	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7843139	M	M	NA	Agree	0.07	12.62	0.00	54.64	17.90	63.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.876174	0.057164	0.043583	0.02308	87.13	4.11	8.49	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7843140	M	M	NA	Agree	0.07	14.46	0.00	57.73	18.81	58.81	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890161	0.054735	0.044255	0.010849	87.74	3.04	8.97	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7843141	M	M	NA	Agree	0.06	12.60	0.00	50.44	16.98	56.72	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.88668	0.04981	0.042718	0.020793	84.90	3.99	10.85	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7843142	M	M	NA	Agree	0.03	11.13	0.00	48.71	17.87	50.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89255	0.050508	0.042219	0.014723	85.54	3.46	10.73	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does