Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7749991	M	M	NA	Agree	0.05	12.29	0.00	58.25	31.92	79.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.871147	0.08481	0.042379	0.001665	89.47	2.81	7.32	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7749992	M	M	NA	Agree	0.01	10.98	0.00	54.00	27.16	76.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.882165	0.07317	0.0425	0.002165	87.27	2.99	9.34	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7749993	M	M	NA	Agree	0.00	7.87	0.00	40.29	23.60	78.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.899566	0.055279	0.042728	0.002427	88.03	3.12	8.49	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7749994	M	M	NA	Agree	0.04	8.52	0.00	42.58	24.60	80.91	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891882	0.064068	0.042587	0.001464	86.93	3.06	9.64	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7749995	M	M	NA	Agree	0.04	11.40	0.00	61.01	29.53	84.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.883226	0.072422	0.042569	0.001784	88.96	2.86	7.77	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7749996	M	M	NA	Agree	0.04	10.34	0.00	57.84	28.70	80.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884713	0.071628	0.042638	0.001021	87.45	2.88	9.26	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7749997	M	M	NA	Agree	0.04	10.27	0.00	52.14	26.09	79.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884072	0.071728	0.042163	0.002037	89.43	3.01	7.16	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7749998	M	M	NA	Agree	0.05	10.69	0.00	61.39	29.23	82.78	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889503	0.067223	0.041557	0.001717	90.26	2.87	6.52	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7749999	M	M	NA	Agree	0.11	10.78	0.00	51.47	25.30	79.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.865681	0.07644	0.041132	0.016746	86.95	4.36	8.28	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750000	M	M	NA	Agree	0.08	12.68	0.00	61.71	28.74	82.99	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.867482	0.083379	0.041435	0.007705	86.49	3.47	9.59	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750001	M	M	NA	Agree	0.04	9.55	0.00	54.54	29.67	75.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.875539	0.0725	0.042399	0.009562	87.34	3.72	8.45	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750002	M	M	NA	Agree	0.08	12.28	0.00	50.26	25.45	76.96	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862128	0.081458	0.041606	0.014809	87.44	4.16	7.99	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750003	M	M	NA	Agree	0.08	13.37	0.00	56.40	33.16	83.36	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873945	0.079925	0.041644	0.004487	88.42	3.13	8.01	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750004	M	M	NA	Agree	0.00	10.58	0.00	52.69	28.18	76.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890244	0.06492	0.041816	0.003021	88.19	3.16	8.22	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750005	M	M	NA	Agree	0.01	10.32	0.00	57.92	27.94	95.28	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.875526	0.07323	0.041925	0.009319	89.04	3.64	6.93	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750006	M	M	NA	Agree	0.02	8.71	0.00	49.24	22.82	78.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903355	0.052611	0.041322	0.002712	90.55	3.26	5.83	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750007	M	M	NA	Agree	0.03	13.90	0.00	62.11	30.60	79.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.875583	0.073628	0.041248	0.009541	88.47	3.60	7.52	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750008	M	M	NA	Agree	0.03	12.27	0.00	65.59	30.00	74.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.882103	0.071121	0.041026	0.00575	89.92	3.28	6.27	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750009	M	M	NA	Agree	0.04	11.73	0.00	61.24	30.59	80.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.877085	0.073681	0.041189	0.008045	89.50	3.40	6.68	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750010	M	M	NA	Agree	0.11	13.73	0.00	57.79	30.04	83.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.881033	0.072344	0.040435	0.006188	89.04	3.26	7.29	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750011	M	M	NA	Agree	0.00	11.23	0.00	52.77	26.10	78.98	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.896691	0.057891	0.040081	0.005337	89.48	3.24	6.87	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750012	M	M	NA	Agree	0.03	10.58	0.00	63.29	29.95	74.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.881809	0.069793	0.041936	0.006462	89.66	3.32	6.56	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750013	M	M	NA	Agree	0.00	10.36	0.00	61.38	30.05	86.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891721	0.064669	0.040959	0.00265	90.63	2.93	6.06	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750014	M	M	NA	Agree	0.00	8.40	0.00	52.05	23.52	72.19	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85024	0.053836	0.038813	0.057111	85.31	8.50	5.81	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750015	M	M	NA	Agree	0.09	13.17	0.00	66.57	31.31	83.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87827	0.075189	0.042863	0.003678	84.59	3.00	11.94	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750016	M	M	NA	Agree	0.03	13.16	0.00	65.71	32.87	85.30	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.860797	0.09007	0.042692	0.006441	87.39	3.18	9.00	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750017	M	M	NA	Agree	0.05	11.44	0.00	51.88	26.64	69.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.785534	0.067026	0.03649	0.11095	77.25	14.41	7.90	145	LP2 (2026-09-30)	PASS	NonPolyA_perM 145 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750018	M	M	NA	Agree	0.02	11.99	0.00	69.20	27.10	81.30	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.883303	0.06962	0.041503	0.005574	88.86	3.28	7.40	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750019	M	M	NA	Agree	0.04	10.65	0.00	58.91	28.81	84.63	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.8947	0.057256	0.042433	0.005611	86.96	3.29	9.36	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750020	M	M	NA	Agree	0.04	12.31	0.00	60.43	29.00	85.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.880115	0.07286	0.041404	0.005621	87.34	3.39	8.83	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750021	M	M	NA	Agree	0.03	12.18	0.00	68.23	30.13	80.52	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.878911	0.07247	0.041347	0.007272	87.23	3.29	9.10	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750022	M	M	NA	Agree	0.05	10.40	0.00	68.99	34.16	76.30	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.874696	0.078784	0.042526	0.003994	87.46	3.17	8.91	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750023	M	M	NA	Agree	0.02	8.76	0.00	49.54	25.24	86.97	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89304	0.065043	0.039872	0.002045	91.39	2.99	5.24	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750024	M	M	NA	Agree	0.00	10.03	0.00	53.84	24.71	75.98	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885076	0.068858	0.039958	0.006108	89.82	3.30	6.48	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750025	M	M	NA	Agree	0.01	6.48	0.00	42.25	21.31	77.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.909916	0.047988	0.040507	0.001589	89.51	3.07	7.07	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750026	M	M	NA	Agree	0.07	8.23	0.00	55.51	24.61	72.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890775	0.061568	0.040314	0.007342	88.06	3.50	8.01	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750027	M	M	NA	Agree	0.00	8.98	0.00	44.02	20.66	75.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.902821	0.052065	0.04105	0.004064	91.50	3.35	4.78	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750028	M	M	NA	Agree	0.02	8.53	0.00	42.96	22.15	82.37	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.908461	0.048611	0.040603	0.002326	89.76	3.15	6.74	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750029	M	M	NA	Agree	0.02	5.32	0.00	30.81	20.08	68.98	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.908359	0.045494	0.041793	0.004354	90.79	3.49	5.37	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750030	M	M	NA	Agree	0.00	5.81	0.00	44.60	18.69	74.43	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.909018	0.048093	0.04074	0.002149	91.08	3.26	5.26	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750031	M	M	NA	Agree	0.02	9.65	0.00	55.60	19.27	81.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.902113	0.052678	0.042471	0.002738	89.65	3.04	6.92	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750032	M	M	NA	Agree	0.05	8.05	0.00	43.61	23.34	78.68	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891123	0.05964	0.041845	0.007391	88.13	3.66	7.79	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750033	M	M	NA	Agree	0.04	10.75	0.00	64.31	27.36	82.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889095	0.059214	0.040866	0.010825	89.29	3.76	6.63	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750034	M	M	NA	Agree	0.03	9.40	0.00	54.55	27.21	79.19	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885669	0.068368	0.042962	0.003001	87.00	3.09	9.50	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750035	M	M	NA	Agree	0.05	10.40	0.00	49.39	23.82	73.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.895514	0.058613	0.042245	0.003628	89.31	3.16	7.09	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750036	M	M	NA	Agree	0.00	10.88	0.00	48.80	25.93	78.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.90004	0.055941	0.041461	0.002558	89.42	3.04	7.12	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750037	M	M	NA	Agree	0.09	10.94	0.00	58.61	28.79	86.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889114	0.065798	0.041083	0.004005	90.24	3.07	6.35	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7750038	M	M	NA	Agree	0.05	10.94	0.00	56.01	26.76	86.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890281	0.065823	0.041794	0.002101	90.90	3.00	5.61	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does