Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7688495	F	F	NA	Agree	168.96	0.00	0.00	0.00	0.05	0.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.870668	0.089748	0.038382	0.001202	95.81	1.42	2.62	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688496	F	F	NA	Agree	208.33	0.00	0.00	0.06	0.00	0.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.863398	0.097696	0.03805	0.000856	95.75	1.39	2.69	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688497	F	F	NA	Agree	231.37	0.00	0.00	0.00	0.00	0.22	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.852553	0.102776	0.042812	0.001859	95.63	1.49	2.70	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688498	F	F	NA	Agree	127.40	0.00	0.00	0.07	0.00	0.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.879544	0.076891	0.040879	0.002685	95.79	1.71	2.34	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688499	F	F	NA	Agree	193.81	0.00	0.00	0.00	0.05	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.860448	0.098226	0.040539	0.000788	95.82	1.39	2.63	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688500	F	F	NA	Agree	179.02	0.14	0.00	0.17	0.04	0.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.847161	0.103388	0.044033	0.005417	95.44	1.93	2.45	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688501	F	F	NA	Agree	193.57	0.00	0.00	0.06	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.857287	0.101005	0.040501	0.001206	95.68	1.50	2.63	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688502	F	F	NA	Agree	188.73	0.00	0.00	0.04	0.00	0.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.853488	0.105562	0.039402	0.001548	95.27	1.53	3.01	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688503	F	F	NA	Agree	156.06	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862845	0.095315	0.040917	0.000923	96.45	1.48	1.91	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688504	F	F	NA	Agree	165.83	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85624	0.098639	0.04074	0.004381	93.57	1.61	4.65	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688505	F	F	NA	Agree	161.07	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85729	0.098919	0.039659	0.004132	93.48	1.58	4.76	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688506	F	F	NA	Agree	171.98	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.844544	0.109501	0.040542	0.005414	93.75	1.73	4.34	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does