Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7688477	not_specified	F	NA	Conflict	115.50	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.909987	0.041871	0.045382	0.00276	94.97	2.27	1.99	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688478	not_specified	F	NA	Conflict	152.55	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.8949	0.053589	0.03994	0.011571	92.74	2.78	3.97	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688479	not_specified	F	NA	Conflict	97.88	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.901885	0.043292	0.04366	0.011164	90.96	2.88	5.57	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688480	not_specified	F	NA	Conflict	51.92	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.916423	0.035482	0.04115	0.006944	92.12	2.44	5.06	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688481	not_specified	M	NA	Conflict	0.01	5.57	0.00	21.91	9.21	59.35	NONE	POLYA	0.911171	0.041513	0.039278	0.008038	91.88	2.79	5.02	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688482	not_specified	F	NA	Conflict	105.39	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.903381	0.046934	0.043385	0.0063	91.14	2.35	6.09	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688483	not_specified	F	NA	Conflict	116.51	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.92757	0.035085	0.034591	0.002754	95.74	1.77	2.21	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688484	not_specified	F	NA	Conflict	56.55	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.920499	0.033411	0.042859	0.003231	92.21	2.06	5.28	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688485	not_specified	F	NA	Conflict	105.26	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.909587	0.042092	0.044473	0.003848	93.03	2.25	4.16	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7688486	not_specified	M	NA	Conflict	0.04	6.54	0.00	22.55	10.02	74.61	NONE	POLYA	0.929819	0.027186	0.040234	0.00276	94.64	2.43	2.67	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does