Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7665131	M	M	NA	Agree	0.02	10.30	0.00	39.85	18.01	95.35	NONE	POLYA	0.874087	0.076529	0.039476	0.009908	92.89	2.96	3.90	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665132	M	M	NA	Agree	0.03	11.24	0.00	51.37	20.05	111.45	NONE	POLYA	0.845325	0.110724	0.039603	0.004349	93.12	2.34	4.24	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665133	M	M	NA	Agree	0.09	7.19	0.00	48.67	15.12	106.02	NONE	POLYA	0.856903	0.09068	0.037506	0.014911	92.53	3.44	3.77	129	LP2 (2026-09-30)	PASS	NonPolyA_perM 129 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665134	M	M	NA	Agree	0.03	8.27	0.00	39.36	17.79	83.84	NONE	POLYA	0.862196	0.088188	0.039765	0.009851	92.67	2.89	4.20	133	LP2 (2026-09-30)	PASS	NonPolyA_perM 133 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665135	M	M	NA	Agree	0.03	9.26	0.00	42.29	17.45	90.78	NONE	POLYA	0.882542	0.072436	0.039301	0.005722	93.08	2.47	4.21	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665136	M	M	NA	Agree	0.03	11.06	0.00	45.19	18.06	101.43	NONE	POLYA	0.855967	0.08684	0.040892	0.016301	91.82	3.47	4.41	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665137	M	M	NA	Agree	0.03	8.09	0.00	39.17	17.17	90.16	NONE	POLYA	0.850244	0.099494	0.038352	0.011911	92.67	3.29	3.75	129	LP2 (2026-09-30)	PASS	NonPolyA_perM 129 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665138	M	M	NA	Agree	0.07	8.94	0.00	45.98	17.13	96.68	NONE	POLYA	0.86499	0.08992	0.039768	0.005322	93.42	2.48	3.83	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665139	M	M	NA	Agree	0.03	9.23	0.00	45.64	18.41	98.17	NONE	POLYA	0.862031	0.09363	0.039582	0.004756	93.51	2.52	3.67	148	LP2 (2026-09-30)	PASS	NonPolyA_perM 148 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665140	M	M	NA	Agree	0.04	7.84	0.00	57.31	20.78	90.25	NONE	POLYA	0.877391	0.079887	0.037958	0.004763	92.32	2.71	4.61	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665141	M	M	NA	Agree	0.07	6.88	0.00	40.58	17.81	84.55	NONE	POLYA	0.884682	0.072505	0.038323	0.00449	93.40	2.46	3.89	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665142	M	M	NA	Agree	0.04	10.76	0.00	40.74	15.47	67.79	NONE	POLYA	0.846284	0.100032	0.038038	0.015646	92.63	3.01	4.18	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665143	M	M	NA	Agree	0.04	9.25	0.00	36.02	11.66	72.58	NONE	POLYA	0.876511	0.067665	0.03601	0.019813	92.64	3.30	3.91	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665144	M	M	NA	Agree	0.03	10.17	0.00	41.54	14.29	69.97	NONE	POLYA	0.879689	0.079	0.035921	0.00539	93.94	2.07	3.82	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665145	M	M	NA	Agree	0.02	9.33	0.00	38.58	13.56	68.41	NONE	POLYA	0.878157	0.077644	0.03744	0.006759	93.83	2.26	3.71	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665146	M	M	NA	Agree	0.00	10.78	0.00	39.05	16.31	102.16	NONE	POLYA	0.898436	0.061616	0.034573	0.005375	94.16	2.23	3.40	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665147	M	M	NA	Agree	0.03	9.78	0.00	40.95	13.80	89.06	NONE	POLYA	0.871095	0.073009	0.036546	0.01935	92.54	3.42	3.85	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665148	M	M	NA	Agree	0.02	9.53	0.00	42.49	16.72	89.37	NONE	POLYA	0.873756	0.079137	0.037192	0.009915	93.36	2.56	3.87	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665149	M	M	NA	Agree	0.02	11.17	0.00	45.24	15.07	77.94	NONE	POLYA	0.866037	0.080504	0.037101	0.016357	93.10	3.11	3.60	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665150	M	M	NA	Agree	0.06	10.30	0.00	40.94	14.01	71.51	NONE	POLYA	0.88092	0.078199	0.035877	0.005003	93.91	2.01	3.90	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665151	M	M	NA	Agree	0.03	9.03	0.00	38.61	14.29	69.95	NONE	POLYA	0.880612	0.074127	0.036179	0.009081	93.48	2.42	3.93	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665152	M	M	NA	Agree	0.07	9.69	0.00	39.28	11.57	65.33	NONE	POLYA	0.871706	0.06827	0.035335	0.024689	91.98	3.85	4.00	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665153	M	M	NA	Agree	0.03	11.14	0.00	45.45	15.16	67.12	NONE	POLYA	0.872483	0.077279	0.037244	0.012993	92.83	2.77	4.22	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665154	M	M	NA	Agree	0.05	8.53	0.00	29.52	11.07	62.62	NONE	POLYA	0.886296	0.068288	0.040084	0.005331	94.26	2.17	3.39	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665155	M	M	NA	Agree	0.00	5.81	0.00	28.46	10.01	59.31	NONE	POLYA	0.890234	0.06121	0.03727	0.011286	92.80	2.86	4.16	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665156	M	M	NA	Agree	0.03	6.09	0.00	28.91	10.14	67.29	NONE	POLYA	0.908083	0.046939	0.033298	0.011681	93.47	2.77	3.58	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665157	M	M	NA	Agree	0.01	6.45	0.00	27.12	10.21	59.67	NONE	POLYA	0.885102	0.064846	0.036334	0.013717	93.00	2.98	3.85	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665158	M	M	NA	Agree	0.13	8.58	0.00	33.82	15.05	100.07	NONE	POLYA	0.881557	0.080284	0.034119	0.004041	93.95	2.12	3.73	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665159	M	M	NA	Agree	0.03	8.60	0.00	35.79	13.80	104.20	NONE	POLYA	0.899826	0.062976	0.032939	0.004259	94.49	2.03	3.29	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665160	M	M	NA	Agree	0.05	10.21	0.00	43.12	14.67	101.13	NONE	POLYA	0.876727	0.082055	0.034254	0.006964	94.03	2.35	3.41	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665161	M	M	NA	Agree	0.06	9.02	0.00	37.43	13.08	64.30	NONE	POLYA	0.886817	0.071786	0.037113	0.004284	93.90	2.08	3.84	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665162	M	M	NA	Agree	0.01	10.04	0.00	39.13	14.40	71.66	NONE	POLYA	0.882038	0.077883	0.036392	0.003687	94.53	1.91	3.39	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665163	M	M	NA	Agree	0.05	7.44	0.00	28.75	9.87	68.76	NONE	POLYA	0.89315	0.058397	0.034819	0.013635	93.39	2.94	3.49	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665164	M	M	NA	Agree	0.10	11.06	0.00	46.85	17.73	89.17	NONE	POLYA	0.86505	0.090309	0.039465	0.005176	93.99	2.38	3.40	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665165	M	M	NA	Agree	0.04	9.68	0.00	41.93	15.16	86.21	NONE	POLYA	0.872076	0.077461	0.037044	0.013419	92.92	3.14	3.73	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665166	M	M	NA	Agree	0.06	7.36	0.00	42.63	15.11	79.07	NONE	POLYA	0.889091	0.064886	0.036388	0.009636	93.30	2.70	3.79	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665167	M	M	NA	Agree	0.03	8.88	0.00	37.83	16.48	76.93	NONE	POLYA	0.87844	0.076015	0.038474	0.007071	93.27	2.55	3.94	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665168	M	M	NA	Agree	0.01	11.43	0.00	50.09	18.95	87.60	NONE	POLYA	0.873115	0.083779	0.038807	0.004299	94.06	2.19	3.53	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665169	M	M	NA	Agree	0.03	6.96	0.00	38.16	14.17	81.05	NONE	POLYA	0.888504	0.061839	0.035883	0.013774	92.58	3.05	4.17	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665170	M	M	NA	Agree	0.08	10.96	0.00	41.94	16.14	83.10	NONE	POLYA	0.873907	0.075579	0.038133	0.01238	93.12	3.05	3.61	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665171	M	M	NA	Agree	0.04	8.60	0.00	38.42	13.47	89.55	NONE	POLYA	0.897406	0.055411	0.036171	0.011011	93.56	2.90	3.33	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665172	M	M	NA	Agree	0.03	8.36	0.00	36.81	17.65	82.93	NONE	POLYA	0.872105	0.079347	0.039208	0.00934	93.15	2.65	3.98	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665173	M	M	NA	Agree	0.00	8.61	0.00	35.06	12.65	79.37	NONE	POLYA	0.894381	0.058522	0.036229	0.010868	92.99	2.82	3.98	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7665174	M	M	NA	Agree	0.03	9.66	0.00	41.56	17.25	101.05	NONE	POLYA	0.896364	0.05841	0.036615	0.008612	93.37	2.74	3.68	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does