Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7655774	M	M	NA	Agree	0.03	7.31	0.00	47.26	16.63	144.13	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873489	0.066675	0.052421	0.007415	70.13	2.33	27.31	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655775	M	M	NA	Agree	0.05	7.62	0.00	47.08	14.93	137.46	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.870633	0.064986	0.05251	0.011871	68.73	2.70	28.32	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655776	M	M	NA	Agree	0.08	6.71	0.00	46.73	17.29	135.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.874356	0.066214	0.053256	0.006173	69.27	2.20	28.28	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655777	M	M	NA	Agree	0.03	7.02	0.00	38.57	13.72	137.31	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.880033	0.057321	0.051958	0.010688	66.46	2.62	30.68	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655778	M	M	NA	Agree	0.01	7.24	0.00	41.02	14.63	126.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87461	0.067268	0.052836	0.005286	72.74	2.31	24.68	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655779	M	M	NA	Agree	0.00	10.96	0.00	54.87	16.93	160.97	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864669	0.072049	0.055374	0.007908	62.21	2.33	35.20	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655780	M	M	NA	Agree	0.09	8.53	0.00	45.45	15.36	137.01	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868645	0.071583	0.0535	0.006273	65.93	2.20	31.61	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655781	M	M	NA	Agree	0.09	7.78	0.00	42.76	15.90	121.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87204	0.069319	0.051359	0.007283	70.32	2.47	26.95	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655782	M	M	NA	Agree	0.08	8.60	0.00	48.47	18.02	127.91	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864369	0.073252	0.052837	0.009543	67.14	2.43	30.20	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655783	M	M	NA	Agree	0.12	7.74	0.00	48.78	15.85	129.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869174	0.070714	0.050861	0.009252	71.15	2.52	26.09	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655784	M	M	NA	Agree	0.16	6.57	0.00	45.11	14.84	136.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.866352	0.071537	0.05214	0.009971	70.77	2.63	26.35	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655785	M	M	NA	Agree	0.10	7.79	0.00	47.89	15.55	141.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864332	0.06975	0.052285	0.013633	68.41	2.90	28.44	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655786	M	M	NA	Agree	0.05	8.68	0.00	53.50	16.99	141.63	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85774	0.079212	0.05187	0.011178	68.03	2.76	28.96	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655787	M	M	NA	Agree	0.03	8.01	0.00	47.60	14.80	129.72	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862524	0.073665	0.052517	0.011295	69.92	2.75	27.07	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655788	M	M	NA	Agree	0.03	7.42	0.00	49.65	15.95	121.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.863548	0.073188	0.053222	0.010042	71.94	2.66	25.14	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7655789	M	M	NA	Agree	0.08	10.04	0.00	51.03	17.05	129.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.853173	0.081465	0.052033	0.013329	68.62	2.95	28.17	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does