Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7578317	F	F	NA	Agree	151.05	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.853884	0.09692	0.042595	0.006601	91.50	1.99	6.27	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578318	F	F	NA	Agree	139.88	0.00	0.00	0.05	0.00	0.00	NONE	POLYA	0.845777	0.106522	0.043051	0.00465	91.29	1.80	6.68	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578319	F	F	NA	Agree	132.51	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.850701	0.101333	0.042642	0.005324	92.31	1.84	5.63	114	LP2 (2026-09-30)	PASS	NonPolyA_perM 114 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578320	F	F	NA	Agree	221.25	0.00	0.00	0.05	0.00	0.00	NONE	POLYA	0.832392	0.118183	0.045179	0.004245	91.57	1.80	6.37	151	LP2 (2026-09-30)	PASS	NonPolyA_perM 151 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578321	F	F	NA	Agree	139.10	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.855727	0.092383	0.044292	0.007598	91.74	2.13	5.90	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578322	F	F	NA	Agree	153.06	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.845364	0.106498	0.043667	0.004472	90.45	1.78	7.53	120	LP2 (2026-09-30)	PASS	NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578323	F	F	NA	Agree	197.51	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.840558	0.110058	0.04445	0.004934	92.07	1.85	5.84	141	LP2 (2026-09-30)	PASS	NonPolyA_perM 141 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578324	F	F	NA	Agree	145.53	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.851052	0.099184	0.04353	0.006235	92.16	1.95	5.66	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578325	F	F	NA	Agree	144.92	0.00	0.00	0.05	0.00	0.00	NONE	POLYA	0.854882	0.095839	0.042407	0.006872	91.25	2.00	6.52	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578326	F	F	NA	Agree	180.54	0.00	0.00	0.06	0.00	0.11	NONE	POLYA	0.838728	0.112695	0.044444	0.004133	91.10	1.80	6.84	130	LP2 (2026-09-30)	PASS	NonPolyA_perM 130 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578327	F	F	NA	Agree	180.73	0.00	0.00	0.06	0.00	0.00	NONE	POLYA	0.842175	0.107714	0.044803	0.005308	91.75	1.91	6.09	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578328	F	F	NA	Agree	194.74	0.00	0.00	0.05	0.00	0.10	NONE	POLYA	0.84115	0.108488	0.045453	0.004908	91.30	1.88	6.57	142	LP2 (2026-09-30)	PASS	NonPolyA_perM 142 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578329	F	F	NA	Agree	175.75	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.838673	0.11244	0.044453	0.004434	91.16	1.83	6.77	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578330	F	F	NA	Agree	165.53	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.810326	0.121654	0.044258	0.023762	89.90	3.65	6.18	144	LP2 (2026-09-30)	PASS	NonPolyA_perM 144 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578331	F	F	NA	Agree	156.63	0.00	0.00	0.00	0.00	0.10	NONE	POLYA	0.854627	0.096547	0.043047	0.005779	91.76	1.90	6.11	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7578332	F	F	NA	Agree	158.66	0.00	0.00	0.00	0.00	0.11	NONE	POLYA	0.848108	0.102501	0.044224	0.005167	91.45	1.87	6.43	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does