Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7383741	F	F	NA	Agree	46.14	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.912717	0.033575	0.03345	0.020258	95.06	3.23	1.60	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383742	F	F	NA	Agree	63.97	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.905169	0.037041	0.034648	0.023142	94.43	3.38	2.08	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383743	F	F	NA	Agree	57.32	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.906348	0.035985	0.034734	0.022932	94.93	3.41	1.56	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383744	F	F	NA	Agree	46.74	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.905461	0.034234	0.032296	0.028009	94.24	3.95	1.71	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383745	F	F	NA	Agree	52.10	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.905335	0.034437	0.034626	0.025603	94.48	3.61	1.80	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383746	F	F	NA	Agree	49.99	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.908199	0.034227	0.033715	0.023859	94.41	3.43	2.05	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383747	F	F	NA	Agree	40.66	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.917437	0.031335	0.031788	0.019441	94.83	3.12	1.94	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383748	F	F	NA	Agree	36.07	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.912338	0.028638	0.033452	0.025571	94.39	3.65	1.85	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383749	F	F	NA	Agree	36.60	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.916078	0.030938	0.032329	0.020655	94.88	3.18	1.84	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383750	F	F	NA	Agree	58.16	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.9096	0.034842	0.033839	0.021719	94.37	3.23	2.29	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383751	F	F	NA	Agree	84.00	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.90081	0.039404	0.035571	0.024214	94.51	3.51	1.86	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383752	F	F	NA	Agree	98.21	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.89697	0.044944	0.034358	0.023728	94.38	3.42	2.08	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383753	F	F	NA	Agree	260.13	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.784747	0.126566	0.042188	0.046499	92.07	5.49	2.25	120	LP2 (2026-09-30)	PASS	NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383754	F	F	NA	Agree	223.64	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.805607	0.112412	0.042327	0.039654	93.56	4.72	1.55	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383755	F	F	NA	Agree	221.59	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.795401	0.114924	0.043704	0.045971	92.40	5.65	1.79	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383756	F	F	NA	Agree	230.41	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.786873	0.119241	0.042035	0.051851	91.81	5.86	2.14	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383757	F	F	NA	Agree	237.73	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.7642	0.118897	0.041936	0.074966	89.23	8.15	2.42	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383758	F	F	NA	Agree	132.18	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.819407	0.081078	0.039719	0.059796	90.72	6.65	2.47	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383759	F	F	NA	Agree	215.36	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.803713	0.101706	0.041705	0.052876	91.49	6.11	2.23	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7383760	F	F	NA	Agree	251.22	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.765721	0.123058	0.042816	0.068405	89.85	7.49	2.47	114	LP2 (2026-09-30)	PASS	NonPolyA_perM 114 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does