Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7291678	M	M	NA	Agree	0.00	8.96	0.00	57.07	20.35	73.37	NONE	POLYA	0.908964	0.047601	0.041981	0.001454	76.56	1.62	21.61	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7291679	M	M	NA	Agree	0.02	11.64	0.00	62.23	16.62	72.66	NONE	POLYA	0.899442	0.054986	0.0426	0.002972	78.45	1.73	19.61	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7291680	M	M	NA	Agree	0.00	8.99	0.00	64.53	20.56	75.54	NONE	POLYA	0.894611	0.058635	0.045234	0.00152	80.00	1.61	18.17	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7291681	M	M	NA	Agree	0.00	9.97	0.00	58.89	18.60	73.68	NONE	POLYA	0.896981	0.058197	0.04335	0.001473	79.63	1.62	18.54	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7291682	M	M	NA	Agree	0.01	6.03	0.00	48.82	14.23	77.09	NONE	POLYA	0.913123	0.044408	0.041434	0.001035	81.83	1.60	16.39	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7291683	M	M	NA	Agree	0.09	7.34	0.00	50.32	18.47	77.32	NONE	POLYA	0.888152	0.066276	0.043745	0.001827	81.57	1.64	16.58	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7291684	M	M	NA	Agree	0.01	7.95	0.00	50.13	16.51	74.93	NONE	POLYA	0.893018	0.061089	0.043585	0.002309	78.30	1.64	19.86	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7291685	M	M	NA	Agree	0.05	7.29	0.00	53.62	18.25	80.27	NONE	POLYA	0.89832	0.056859	0.0432	0.00162	83.04	1.66	15.09	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does