Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7234204	M	M	NA	Agree	0.00	4.35	0.00	18.78	5.84	34.35	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.75837	0.142015	0.085943	0.013672	90.70	6.74	2.14	1073	LP2 (2026-09-30)	PASS	NonPolyA_perM 1073 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7234205	M	M	NA	Agree	0.00	4.38	0.00	22.81	5.94	46.81	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.814204	0.097796	0.082816	0.005185	91.78	6.07	1.80	1158	LP2 (2026-09-30)	PASS	NonPolyA_perM 1158 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7234206	M	M	NA	Agree	0.00	5.14	0.00	21.94	5.82	45.19	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.814606	0.100746	0.082477	0.002171	92.30	5.46	1.90	928	LP2 (2026-09-30)	PASS	NonPolyA_perM 928 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7234207	M	M	NA	Agree	0.01	2.62	0.00	13.38	3.85	17.96	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.662031	0.226184	0.106212	0.005573	85.74	8.27	5.45	3993	LP2 (2026-09-30)	PASS	NonPolyA_perM 3993 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7234208	M	M	NA	Agree	0.01	3.09	0.00	15.82	4.18	23.10	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.696502	0.204706	0.095281	0.003511	87.42	7.02	5.08	2745	LP2 (2026-09-30)	PASS	NonPolyA_perM 2745 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7234209	M	M	NA	Agree	0.00	2.24	0.00	14.83	3.46	19.63	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.750972	0.137084	0.108078	0.003867	84.96	9.20	5.42	3975	LP2 (2026-09-30)	PASS	NonPolyA_perM 3975 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7234210	M	M	NA	Agree	0.01	4.83	0.00	33.27	8.24	39.35	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.787311	0.093081	0.062354	0.057254	86.98	9.91	2.77	1996	LP2 (2026-09-30)	PASS	NonPolyA_perM 1996 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7234211	M	M	NA	Agree	0.01	1.86	0.00	10.07	2.73	16.83	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.739993	0.133546	0.120662	0.0058	87.32	10.76	1.58	2230	LP2 (2026-09-30)	PASS	NonPolyA_perM 2230 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7234212	M	M	NA	Agree	0.00	3.26	0.00	18.50	5.38	29.12	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.740613	0.160915	0.0944	0.004073	91.58	6.55	1.51	1417	LP2 (2026-09-30)	PASS	NonPolyA_perM 1417 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7234213	M	Undetermined	NA	Conflict	14.05	3.16	0.00	9.56	8.20	21.88	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.828648	0.093939	0.075369	0.002044	93.26	4.86	1.49	822	LP2 (2026-09-30)	PASS	NonPolyA_perM 822 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7234214	M	M	NA	Agree	0.03	8.34	0.00	26.60	13.93	58.83	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.763955	0.166349	0.068615	0.001081	94.36	3.43	1.72	549	LP2 (2026-09-30)	PASS	NonPolyA_perM 549 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM7234215	M	M	NA	Agree	0.00	5.69	0.00	20.30	10.14	45.30	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.827976	0.102615	0.067853	0.001556	94.04	3.87	1.72	477	LP2 (2026-09-30)	PASS	NonPolyA_perM 477 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM7234216	M	M	NA	Agree	0.01	6.77	0.00	30.74	11.19	61.91	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.847767	0.080919	0.068135	0.003179	94.03	3.82	1.85	644	LP2 (2026-09-30)	PASS	NonPolyA_perM 644 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM7234217	M	M	NA	Agree	0.01	7.03	0.00	34.19	9.37	65.95	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.827464	0.103601	0.067564	0.001371	93.57	4.14	1.90	653	LP2 (2026-09-30)	PASS	NonPolyA_perM 653 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM7234218	M	M	NA	Agree	0.01	4.36	0.00	21.84	7.87	52.30	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.850132	0.072866	0.073353	0.00365	92.11	5.08	2.41	1113	LP2 (2026-09-30)	PASS	NonPolyA_perM 1113 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not