Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7154228	M	M	NA	Agree	0.00	5.87	0.00	25.38	5.02	31.47	NONE	POLYA	0.935815	0.037009	0.022224	0.004952	93.16	1.76	4.98	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7154229	M	M	NA	Agree	0.00	6.29	0.00	31.21	7.69	28.56	NONE	POLYA	0.93261	0.041555	0.018412	0.007423	94.54	2.26	3.11	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7154230	M	M	NA	Agree	0.00	4.21	0.00	32.29	6.12	32.14	NONE	POLYA	0.938352	0.033666	0.016054	0.011928	95.00	2.24	2.68	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7154231	M	M	NA	Agree	0.00	3.54	0.00	21.15	7.17	42.15	NONE	POLYA	0.90611	0.042679	0.031509	0.019701	93.58	3.45	2.84	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7154232	M	M	NA	Agree	0.00	5.34	0.00	21.40	6.96	45.24	NONE	POLYA	0.902946	0.04461	0.030469	0.021975	90.79	4.24	4.58	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7154233	M	M	NA	Agree	0.00	4.18	0.00	17.32	5.95	44.51	NONE	POLYA	0.903971	0.043844	0.029537	0.022647	93.09	3.94	2.81	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7154234	M	M	NA	Agree	0.00	2.49	0.00	12.46	2.68	41.45	NONE	POLYA	0.927452	0.02913	0.026008	0.01741	94.18	3.26	2.44	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7154235	M	M	NA	Agree	0.00	4.09	0.00	12.17	4.62	39.53	NONE	POLYA	0.930415	0.034045	0.026878	0.008663	94.85	2.42	2.61	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7154236	M	M	NA	Agree	0.00	4.61	0.00	19.90	7.17	45.05	NONE	POLYA	0.912492	0.040127	0.030498	0.016882	92.03	3.26	4.54	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does