Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7142542	M	F	NA	Conflict	20.19	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.911172	0.028582	0.041798	0.018448	67.64	2.96	29.22	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142543	M	F	NA	Conflict	26.83	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.919936	0.029376	0.042932	0.007756	68.09	2.02	29.69	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142544	M	F	NA	Conflict	24.70	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.917972	0.027642	0.042332	0.012054	66.83	2.42	30.57	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142545	M	F	NA	Conflict	26.04	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.918164	0.029495	0.041341	0.011001	66.44	2.29	31.08	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142546	M	F	NA	Conflict	28.31	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.90447	0.03491	0.040917	0.019704	65.91	3.14	30.76	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142547	M	F	NA	Conflict	31.34	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.919034	0.029182	0.041305	0.010479	67.25	2.32	30.26	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142548	F	M	NA	Conflict	0.19	2.90	0.00	22.01	7.81	36.64	NONE	POLYA	0.91963	0.027975	0.040843	0.011552	66.59	2.39	30.85	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142549	F	M	NA	Conflict	0.07	4.32	0.00	20.57	10.87	43.27	NONE	POLYA	0.916385	0.032889	0.041992	0.008733	66.59	2.13	31.09	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142550	F	M	NA	Conflict	0.07	3.01	0.00	20.37	8.46	43.81	NONE	POLYA	0.912081	0.037383	0.04239	0.008145	66.24	2.08	31.49	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142551	F	M	NA	Conflict	0.02	2.83	0.00	24.78	9.14	50.02	NONE	POLYA	0.91001	0.036198	0.043765	0.010026	65.30	2.25	32.25	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142552	F	M	NA	Conflict	0.06	3.56	0.00	23.50	9.65	40.66	NONE	POLYA	0.913218	0.031016	0.043338	0.012428	65.04	2.41	32.37	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142553	F	M	NA	Conflict	0.00	3.55	0.00	20.67	9.12	42.45	NONE	POLYA	0.894509	0.037654	0.042871	0.024966	62.90	3.55	33.36	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142554	M	F	NA	Conflict	26.98	0.00	0.00	0.00	0.00	0.19	NONE	POLYA	0.920145	0.028685	0.041897	0.009274	66.71	2.15	30.95	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142555	M	F	NA	Conflict	34.07	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.911224	0.033837	0.041927	0.013011	64.40	2.42	33.00	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142556	M	F	NA	Conflict	41.82	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.911395	0.037234	0.042255	0.009116	64.90	2.09	32.80	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142557	M	F	NA	Conflict	33.22	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.916109	0.029427	0.041793	0.012671	65.65	2.37	31.80	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142558	M	F	NA	Conflict	37.79	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.917134	0.028896	0.041746	0.012224	67.82	2.41	29.60	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142559	M	F	NA	Conflict	36.22	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.920329	0.030515	0.042065	0.00709	68.50	1.95	29.37	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142560	F	M	NA	Conflict	0.00	3.70	0.00	24.51	10.72	40.85	NONE	POLYA	0.916722	0.03415	0.042719	0.006409	66.68	1.90	31.21	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142561	F	M	NA	Conflict	0.03	3.26	0.00	23.70	9.23	34.15	NONE	POLYA	0.912503	0.034198	0.043129	0.01017	67.18	2.26	30.36	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142562	F	M	NA	Conflict	0.07	3.55	0.00	24.05	10.19	43.00	NONE	POLYA	0.913226	0.034566	0.042062	0.010145	66.60	2.18	31.04	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142563	F	M	NA	Conflict	0.05	3.16	0.00	27.26	12.29	45.61	NONE	POLYA	0.916455	0.033141	0.042429	0.007975	65.44	1.96	32.41	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142564	F	M	NA	Conflict	0.12	3.11	0.00	25.00	10.02	43.04	NONE	POLYA	0.91833	0.031086	0.043043	0.007542	66.25	2.04	31.50	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7142565	F	M	NA	Conflict	0.03	2.81	0.00	25.66	12.06	50.30	NONE	POLYA	0.911829	0.038266	0.043052	0.006853	66.85	1.91	31.06	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does