Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7100741	F	F	NA	Agree	176.32	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.537381	0.381241	0.080406	0.000972	92.05	5.49	1.84	2371	LP2 (2026-09-30)	PASS	NonPolyA_perM 2371 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7100742	F	F	NA	Agree	178.72	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.537261	0.38115	0.080353	0.001236	92.46	5.26	1.69	2315	LP2 (2026-09-30)	PASS	NonPolyA_perM 2315 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7100743	F	F	NA	Agree	190.66	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.562694	0.356617	0.079953	0.000736	92.45	5.35	1.47	2739	LP2 (2026-09-30)	PASS	NonPolyA_perM 2739 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7100744	F	F	NA	Agree	152.02	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.646719	0.278327	0.073229	0.001725	92.98	5.02	1.42	1945	LP2 (2026-09-30)	PASS	NonPolyA_perM 1945 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7100745	F	F	NA	Agree	148.79	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.652681	0.27021	0.075194	0.001915	92.67	5.30	1.44	2065	LP2 (2026-09-30)	PASS	NonPolyA_perM 2065 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7100746	F	F	NA	Agree	152.92	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.647578	0.276388	0.074078	0.001956	92.88	5.16	1.36	2078	LP2 (2026-09-30)	PASS	NonPolyA_perM 2078 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7100747	F	F	NA	Agree	201.82	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.570267	0.360031	0.068016	0.001685	93.61	4.24	1.55	2045	LP2 (2026-09-30)	PASS	NonPolyA_perM 2045 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7100748	F	F	NA	Agree	173.15	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.610476	0.316211	0.071407	0.001906	93.24	4.58	1.58	2072	LP2 (2026-09-30)	PASS	NonPolyA_perM 2072 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7100749	F	F	NA	Agree	193.13	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.59111	0.338633	0.068906	0.001351	93.29	4.32	1.70	2525	LP2 (2026-09-30)	PASS	NonPolyA_perM 2525 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7100750	F	F	NA	Agree	198.20	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.558427	0.366973	0.073362	0.001238	93.15	4.51	1.75	2033	LP2 (2026-09-30)	PASS	NonPolyA_perM 2033 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7100751	F	F	NA	Agree	222.87	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.528322	0.398378	0.07236	0.00094	93.15	4.44	1.81	2095	LP2 (2026-09-30)	PASS	NonPolyA_perM 2095 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7100752	F	F	NA	Agree	226.93	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.537998	0.387075	0.073521	0.001406	93.02	4.35	2.09	1889	LP2 (2026-09-30)	PASS	NonPolyA_perM 1889 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not