Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7094332	M	M	NA	Agree	0.00	3.22	0.00	24.28	10.10	75.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.882184	0.068821	0.038189	0.010806	89.02	4.08	6.70	150	LP2 (2026-09-30)	PASS	NonPolyA_perM 150 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094333	M	M	NA	Agree	0.00	4.57	0.00	28.37	13.59	73.78	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890097	0.07111	0.036864	0.001929	92.76	1.90	5.19	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094334	M	M	NA	Agree	0.00	4.51	0.00	27.08	9.13	74.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.875807	0.067335	0.040686	0.016172	87.05	3.76	9.02	181	LP2 (2026-09-30)	PASS	NonPolyA_perM 181 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094335	M	M	NA	Agree	0.00	5.00	0.00	42.53	12.62	67.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.880546	0.080401	0.036758	0.002295	92.22	2.97	4.61	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094336	M	M	NA	Agree	0.00	6.11	0.00	49.96	15.84	80.80	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885899	0.078249	0.033202	0.00265	92.94	2.01	4.89	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094337	M	M	NA	Agree	0.00	3.18	0.00	35.76	10.64	75.03	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.90415	0.059466	0.034396	0.001988	91.46	1.94	6.47	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094338	M	M	NA	Agree	0.00	3.67	0.00	33.12	9.23	72.98	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.908821	0.056824	0.032647	0.001708	90.70	1.96	7.21	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094339	M	M	NA	Agree	0.00	4.64	0.00	27.89	10.27	72.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.904983	0.059544	0.033895	0.001578	92.19	1.86	5.82	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094340	M	M	NA	Agree	0.00	3.45	0.00	27.64	8.51	67.72	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.906921	0.055593	0.035366	0.00212	91.71	2.21	5.94	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094341	M	M	NA	Agree	0.00	4.16	0.00	29.53	8.89	75.94	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.918804	0.049181	0.030527	0.001487	91.60	1.87	6.42	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094342	M	M	NA	Agree	0.00	4.13	0.00	24.49	11.49	70.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.908847	0.049295	0.030439	0.011419	89.96	2.56	7.38	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094343	M	M	NA	Agree	0.00	2.03	0.00	14.71	8.77	60.58	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.928286	0.028936	0.039346	0.003432	90.86	2.42	6.63	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094344	M	M	NA	Agree	0.00	3.58	0.00	25.23	10.89	73.67	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.904059	0.057681	0.034379	0.003881	92.85	2.09	4.93	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094345	M	M	NA	Agree	0.00	4.28	0.00	25.16	11.28	74.93	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.90919	0.054225	0.034713	0.001872	93.71	1.80	4.37	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7094346	M	M	NA	Agree	0.00	4.23	0.00	26.53	11.66	72.75	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.906536	0.055335	0.034826	0.003303	91.99	2.07	5.81	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does