Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-16) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7091159	M	M	NA	Agree	0.46	7.55	0.00	19.04	11.26	15.97	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.42947	0.352136	0.082807	0.135587	75.02	19.13	3.75	11539	LP2 (2026-09-30)	PASS	NonPolyA_perM 11539 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091160	M	M	NA	Agree	0.47	7.27	0.00	20.72	12.71	18.63	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.428623	0.365414	0.088338	0.117624	76.66	17.86	3.25	10757	LP2 (2026-09-30)	PASS	NonPolyA_perM 10757 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091161	M	M	NA	Agree	0.59	8.89	0.00	25.01	13.76	19.02	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.456186	0.432206	0.094415	0.017192	84.37	8.36	4.54	12855	LP2 (2026-09-30)	PASS	NonPolyA_perM 12855 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091162	M	M	NA	Agree	0.65	7.48	0.00	24.94	16.42	21.63	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.483854	0.419313	0.090198	0.006635	86.44	6.77	4.85	9735	LP2 (2026-09-30)	PASS	NonPolyA_perM 9735 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091163	M	M	NA	Agree	0.52	8.40	0.00	25.92	16.81	19.85	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.462149	0.444271	0.08763	0.00595	86.61	6.18	5.25	9140	LP2 (2026-09-30)	PASS	NonPolyA_perM 9140 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091164	M	M	NA	Agree	0.61	7.69	0.00	20.65	14.15	18.83	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.471511	0.429257	0.091887	0.007345	85.64	6.81	5.48	9212	LP2 (2026-09-30)	PASS	NonPolyA_perM 9212 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091165	M	M	NA	Agree	0.68	9.26	0.00	25.20	14.90	21.24	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.480022	0.425805	0.088176	0.005997	84.88	6.22	6.85	8792	LP2 (2026-09-30)	PASS	NonPolyA_perM 8792 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091166	M	M	NA	Agree	0.64	8.56	0.00	25.58	16.77	22.46	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.482749	0.419901	0.087298	0.010052	84.95	6.88	6.06	9498	LP2 (2026-09-30)	PASS	NonPolyA_perM 9498 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091167	M	M	NA	Agree	0.57	9.33	0.00	24.34	17.16	20.28	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.472999	0.425379	0.090413	0.011208	84.47	6.92	6.65	9274	LP2 (2026-09-30)	PASS	NonPolyA_perM 9274 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091168	M	M	NA	Agree	0.67	9.11	0.00	25.52	14.14	20.08	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.460716	0.438557	0.089128	0.0116	85.11	7.04	5.75	9340	LP2 (2026-09-30)	PASS	NonPolyA_perM 9340 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091169	M	M	NA	Agree	0.57	7.34	0.00	23.20	13.84	17.18	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.470756	0.420614	0.096007	0.012623	85.49	7.77	4.72	10075	LP2 (2026-09-30)	PASS	NonPolyA_perM 10075 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091170	M	M	NA	Agree	0.68	9.94	0.00	25.77	16.16	19.42	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.456912	0.438575	0.088905	0.015608	85.65	7.44	4.97	9557	LP2 (2026-09-30)	PASS	NonPolyA_perM 9557 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091171	M	M	NA	Agree	0.51	7.44	0.00	20.20	12.14	17.72	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.481359	0.419627	0.096202	0.002811	85.21	6.67	6.58	9303	LP2 (2026-09-30)	PASS	NonPolyA_perM 9303 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091172	M	M	NA	Agree	0.66	9.22	0.00	22.67	13.88	15.28	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.447805	0.457179	0.089647	0.005368	85.94	6.45	5.40	10762	LP2 (2026-09-30)	PASS	NonPolyA_perM 10762 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091173	M	M	NA	Agree	0.64	7.71	0.00	22.83	12.51	20.31	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.473411	0.425568	0.093658	0.007363	84.14	7.32	6.26	11934	LP2 (2026-09-30)	PASS	NonPolyA_perM 11934 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091174	M	M	NA	Agree	0.62	8.37	0.00	23.16	13.64	18.97	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.479292	0.416607	0.096095	0.008006	85.02	7.21	5.53	10517	LP2 (2026-09-30)	PASS	NonPolyA_perM 10517 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091175	M	M	NA	Agree	0.56	7.85	0.00	18.82	13.15	17.43	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.461449	0.435367	0.096725	0.00646	84.97	7.15	5.51	12413	LP2 (2026-09-30)	PASS	NonPolyA_perM 12413 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091176	M	M	NA	Agree	0.58	8.26	0.00	25.45	16.63	21.19	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.484199	0.417439	0.089535	0.008827	84.90	6.99	5.86	10303	LP2 (2026-09-30)	PASS	NonPolyA_perM 10303 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091177	M	M	NA	Agree	0.67	10.07	0.00	24.46	14.43	20.17	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.463699	0.439389	0.089739	0.007173	85.83	6.59	5.42	11058	LP2 (2026-09-30)	PASS	NonPolyA_perM 11058 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091178	M	M	NA	Agree	0.54	8.52	0.00	23.68	12.65	16.06	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.465955	0.436204	0.092388	0.005453	86.11	6.69	5.18	9753	LP2 (2026-09-30)	PASS	NonPolyA_perM 9753 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091179	M	M	NA	Agree	0.69	9.78	0.00	28.11	16.11	22.31	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.458259	0.4424	0.092606	0.006735	86.29	6.39	5.29	9332	LP2 (2026-09-30)	PASS	NonPolyA_perM 9332 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091180	M	M	NA	Agree	0.86	10.01	0.00	27.94	15.52	23.66	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.471746	0.435619	0.089818	0.002817	87.24	5.85	4.96	8659	LP2 (2026-09-30)	PASS	NonPolyA_perM 8659 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091181	M	M	NA	Agree	0.48	6.64	0.00	18.97	13.35	15.08	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.458888	0.424162	0.107738	0.009212	83.11	8.59	5.57	14336	LP2 (2026-09-30)	PASS	NonPolyA_perM 14336 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091182	M	M	NA	Agree	0.55	8.48	0.00	18.81	13.22	17.85	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.459331	0.432927	0.098556	0.009186	84.35	7.75	5.34	13930	LP2 (2026-09-30)	PASS	NonPolyA_perM 13930 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091183	M	M	NA	Agree	0.41	8.41	0.00	26.06	16.11	22.74	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.465918	0.425487	0.096707	0.011888	87.53	7.80	2.50	8448	LP2 (2026-09-30)	PASS	NonPolyA_perM 8448 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091184	M	M	NA	Agree	0.56	8.82	0.00	23.08	13.01	19.09	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.453249	0.435468	0.088487	0.022796	87.14	8.51	2.22	8580	LP2 (2026-09-30)	PASS	NonPolyA_perM 8580 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091185	M	M	NA	Agree	0.59	9.45	0.00	24.26	15.23	20.82	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.476622	0.416281	0.093666	0.013431	86.80	8.18	2.78	8915	LP2 (2026-09-30)	PASS	NonPolyA_perM 8915 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091186	M	M	NA	Agree	0.64	8.39	0.00	23.98	13.55	19.70	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.461493	0.43192	0.091773	0.014814	86.77	8.05	3.18	8961	LP2 (2026-09-30)	PASS	NonPolyA_perM 8961 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091187	M	M	NA	Agree	0.47	8.19	0.00	21.73	13.28	18.54	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.454123	0.400048	0.090882	0.054947	82.24	11.80	3.85	9635	LP2 (2026-09-30)	PASS	NonPolyA_perM 9635 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091188	M	M	NA	Agree	0.58	8.63	0.00	24.83	15.31	21.20	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.459045	0.421954	0.095519	0.023482	84.46	8.70	4.77	9320	LP2 (2026-09-30)	PASS	NonPolyA_perM 9320 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091189	M	M	NA	Agree	0.66	9.01	0.00	26.45	15.43	24.14	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.462142	0.419476	0.089546	0.028836	85.59	8.69	3.71	8866	LP2 (2026-09-30)	PASS	NonPolyA_perM 8866 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091190	M	M	NA	Agree	0.89	10.49	0.00	25.34	14.66	15.73	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.401167	0.488316	0.086657	0.02386	86.76	8.18	2.92	9910	LP2 (2026-09-30)	PASS	NonPolyA_perM 9910 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091191	M	M	NA	Agree	0.87	9.52	0.00	23.92	12.69	18.54	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.430432	0.443613	0.089131	0.036825	85.24	9.94	2.82	9368	LP2 (2026-09-30)	PASS	NonPolyA_perM 9368 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091192	M	M	NA	Agree	0.90	8.78	0.00	24.47	12.44	17.87	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.439435	0.441621	0.087806	0.031138	85.19	9.63	3.00	9800	LP2 (2026-09-30)	PASS	NonPolyA_perM 9800 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091193	M	M	NA	Agree	0.87	9.74	0.00	24.29	13.64	17.57	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.414491	0.456232	0.084515	0.044762	84.33	10.17	3.44	9564	LP2 (2026-09-30)	PASS	NonPolyA_perM 9564 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091194	M	M	NA	Agree	0.75	9.52	0.00	23.50	12.04	17.82	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.432104	0.443467	0.091398	0.03303	84.20	9.72	3.96	9946	LP2 (2026-09-30)	PASS	NonPolyA_perM 9946 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091195	M	M	NA	Agree	0.77	9.53	0.00	26.90	14.11	20.99	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.434201	0.435409	0.091494	0.038896	83.69	10.34	3.70	10098	LP2 (2026-09-30)	PASS	NonPolyA_perM 10098 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091196	M	M	NA	Agree	0.78	9.74	0.00	23.81	13.97	19.72	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.408802	0.464042	0.086428	0.040727	83.37	9.40	5.64	9431	LP2 (2026-09-30)	PASS	NonPolyA_perM 9431 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091197	M	M	NA	Agree	0.86	10.65	0.00	27.03	14.53	22.72	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.418178	0.442559	0.083299	0.055964	81.59	10.69	6.18	9496	LP2 (2026-09-30)	PASS	NonPolyA_perM 9496 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091198	M	M	NA	Agree	0.86	12.26	0.00	29.68	15.99	23.69	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.416543	0.457079	0.079399	0.046978	83.29	9.47	5.64	8555	LP2 (2026-09-30)	PASS	NonPolyA_perM 8555 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091199	M	M	NA	Agree	1.14	11.87	0.00	25.02	14.49	15.61	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.346289	0.461967	0.076902	0.114843	75.52	15.25	7.68	11131	LP2 (2026-09-30)	PASS	NonPolyA_perM 11131 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091200	M	M	NA	Agree	1.01	10.77	0.00	27.50	13.65	20.99	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.412416	0.451276	0.083417	0.052891	81.56	10.09	6.77	8693	LP2 (2026-09-30)	PASS	NonPolyA_perM 8693 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7091201	M	M	NA	Agree	0.82	10.15	0.00	24.42	13.87	20.84	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.41099	0.442436	0.082699	0.063875	83.00	11.64	3.92	8333	LP2 (2026-09-30)	PASS	NonPolyA_perM 8333 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not