Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7089911	M	M	NA	Agree	0.00	5.40	0.00	32.64	10.88	40.95	NONE	POLYA	0.916625	0.059818	0.022103	0.001453	94.43	1.45	4.01	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7089912	M	M	NA	Agree	0.01	4.69	0.00	31.16	9.35	35.30	NONE	POLYA	0.924111	0.053885	0.020524	0.001481	94.76	1.43	3.70	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7089913	M	M	NA	Agree	0.01	5.35	0.00	32.43	10.32	51.08	NONE	POLYA	0.918881	0.055322	0.023762	0.002036	94.90	1.48	3.48	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7089914	M	M	NA	Agree	0.03	5.65	0.00	33.57	12.68	70.27	NONE	POLYA	0.896728	0.074552	0.027321	0.0014	94.10	1.74	3.98	143	LP2 (2026-09-30)	PASS	NonPolyA_perM 143 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7089915	M	M	NA	Agree	0.00	2.99	0.00	22.83	9.43	38.26	NONE	POLYA	0.92031	0.056668	0.021729	0.001293	94.91	1.59	3.31	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7089916	M	M	NA	Agree	0.00	5.02	0.00	31.43	9.72	57.38	NONE	POLYA	0.906737	0.065002	0.02695	0.00131	94.38	1.72	3.72	142	LP2 (2026-09-30)	PASS	NonPolyA_perM 142 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does