Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7072892	not_specified	M	NA	Conflict	0.02	10.09	0.00	24.37	13.25	26.98	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.540173	0.383727	0.074752	0.001347	92.52	4.22	2.47	2018	LP2 (2026-09-30)	PASS	NonPolyA_perM 2018 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7072893	not_specified	M	NA	Conflict	0.02	9.43	0.00	26.61	12.54	25.38	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.544856	0.375853	0.077874	0.001417	92.00	4.66	2.52	2275	LP2 (2026-09-30)	PASS	NonPolyA_perM 2275 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7072894	not_specified	M	NA	Conflict	0.03	9.55	0.00	24.59	14.06	25.93	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.519226	0.407207	0.072855	0.000713	92.69	4.10	2.44	2457	LP2 (2026-09-30)	PASS	NonPolyA_perM 2457 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7072895	not_specified	M	NA	Conflict	0.01	8.79	0.00	27.30	12.64	23.72	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.502374	0.422365	0.073869	0.001392	92.46	3.99	2.79	2103	LP2 (2026-09-30)	PASS	NonPolyA_perM 2103 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7072896	not_specified	M	NA	Conflict	0.03	10.53	0.00	30.75	11.84	26.99	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.525658	0.398923	0.074097	0.001321	92.25	3.84	3.12	2435	LP2 (2026-09-30)	PASS	NonPolyA_perM 2435 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7072897	not_specified	M	NA	Conflict	0.02	8.85	0.00	26.71	10.86	24.79	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.538545	0.382201	0.077947	0.001307	91.98	4.51	2.70	2588	LP2 (2026-09-30)	PASS	NonPolyA_perM 2588 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7072898	not_specified	M	NA	Conflict	0.00	8.61	0.00	27.56	10.83	24.01	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.532598	0.387015	0.078579	0.001808	92.29	4.31	2.63	2234	LP2 (2026-09-30)	PASS	NonPolyA_perM 2234 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7072899	not_specified	M	NA	Conflict	0.00	8.95	0.00	26.82	12.69	26.37	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.539873	0.377304	0.081105	0.001718	91.70	4.69	2.82	2163	LP2 (2026-09-30)	PASS	NonPolyA_perM 2163 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7072900	not_specified	M	NA	Conflict	0.02	8.45	0.00	27.61	12.23	27.14	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.535877	0.386613	0.076232	0.001279	92.53	4.18	2.48	2260	LP2 (2026-09-30)	PASS	NonPolyA_perM 2260 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not