Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7042490	M	M	NA	Agree	0.00	1.29	0.00	10.32	2.46	20.77	NONE	POLYA	0.953419	0.017186	0.010386	0.019009	76.40	2.93	20.60	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7042491	M	M	NA	Agree	0.00	4.66	0.00	24.23	6.27	26.56	NONE	POLYA	0.957125	0.029466	0.011554	0.001855	86.27	1.37	12.29	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7042492	M	M	NA	Agree	0.02	5.21	0.00	26.80	3.68	27.81	NONE	POLYA	0.965557	0.020734	0.009988	0.003721	84.88	1.52	13.55	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7042493	M	M	NA	Agree	0.00	0.77	0.00	4.20	1.73	15.71	NONE	POLYA	0.975048	0.009472	0.013183	0.002297	82.40	1.78	15.70	12	LP2 (2026-09-30)	PASS	NonPolyA_perM 12 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7042494	M	F	NA	Conflict	12.23	0.00	0.00	0.03	0.00	0.09	NONE	POLYA	0.966438	0.015248	0.01362	0.004693	77.08	1.78	21.01	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7042495	M	Undetermined	NA	Conflict	8.80	0.00	0.00	0.00	0.00	0.21	NONE	POLYA	0.96996	0.013265	0.012369	0.004405	75.48	1.63	22.77	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does