Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7036228	M	M	NA	Agree	0.03	2.22	0.00	11.91	7.56	48.34	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903953	0.042355	0.04863	0.005063	96.09	2.11	1.66	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036229	M	M	NA	Agree	0.03	1.01	0.00	7.34	5.66	40.78	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.905098	0.040009	0.046771	0.008121	95.51	2.47	1.88	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036230	M	M	NA	Agree	0.04	1.85	0.00	8.13	5.91	53.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.907485	0.039343	0.049415	0.003756	95.88	2.12	1.86	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036231	M	M	NA	Agree	0.01	1.91	0.00	10.23	6.28	46.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.905088	0.042538	0.047473	0.004902	96.01	2.10	1.75	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036232	M	M	NA	Agree	0.04	2.14	0.00	13.02	8.11	53.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.899452	0.045953	0.051129	0.003466	96.01	2.05	1.77	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036233	M	M	NA	Agree	0.02	2.24	0.00	13.19	8.83	54.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.902034	0.044501	0.049455	0.004009	96.19	1.99	1.66	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036234	M	M	NA	Agree	0.01	1.85	0.00	10.94	6.89	47.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.905659	0.042439	0.048588	0.003314	96.08	1.97	1.80	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036235	M	M	NA	Agree	0.03	1.63	0.00	11.32	6.94	54.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.90344	0.043528	0.04944	0.003592	96.19	1.99	1.69	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does