Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6964956	F	F	NA	Agree	16.21	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.912985	0.028223	0.040312	0.018481	94.46	3.11	2.33	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6964957	F	F	NA	Agree	48.67	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.909215	0.02955	0.03332	0.027914	93.98	3.97	1.94	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6964958	F	F	NA	Agree	57.89	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.906497	0.032789	0.03463	0.026084	93.96	3.56	2.38	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6964959	F	F	NA	Agree	60.29	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.905368	0.031404	0.034475	0.028753	93.98	4.01	1.90	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6964960	F	F	NA	Agree	77.22	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.872301	0.035775	0.035523	0.056401	91.21	6.59	2.10	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6964961	F	F	NA	Agree	65.71	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.902361	0.038312	0.03537	0.023958	94.15	3.57	2.17	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6964962	F	F	NA	Agree	57.64	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.909461	0.034097	0.034259	0.022183	94.49	3.41	2.01	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6964963	F	F	NA	Agree	82.32	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.908354	0.037545	0.035102	0.018999	94.56	2.96	2.37	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6964964	F	F	NA	Agree	70.20	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.907315	0.037388	0.036114	0.019184	95.17	3.14	1.60	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6964965	F	F	NA	Agree	91.92	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.895652	0.043052	0.037511	0.023784	94.39	3.48	2.02	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does