Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6744228	M	M	NA	Agree	0.01	3.02	0.00	20.29	5.12	33.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.935341	0.030191	0.02368	0.010788	94.79	3.36	1.67	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744229	M	M	NA	Agree	0.02	4.07	0.00	24.80	5.50	42.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.938041	0.026816	0.021862	0.013281	95.22	2.90	1.74	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744230	M	M	NA	Agree	0.01	2.79	0.00	17.77	4.55	27.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.935934	0.028105	0.02211	0.013851	93.90	3.89	2.00	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744231	M	M	NA	Agree	0.01	3.22	0.00	18.69	5.05	30.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.933612	0.032398	0.023227	0.010762	94.57	3.63	1.58	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744232	M	M	NA	Agree	0.02	2.93	0.00	19.77	5.11	34.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.936309	0.030015	0.023454	0.010223	94.71	3.32	1.79	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744233	M	M	NA	Agree	0.00	3.30	0.00	23.63	5.40	35.73	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.933019	0.031879	0.020796	0.014306	95.34	2.84	1.64	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744234	M	M	NA	Agree	0.01	3.35	0.00	18.64	5.15	35.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.930156	0.03308	0.024059	0.012706	94.72	3.47	1.65	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744235	M	M	NA	Agree	0.01	2.49	0.00	17.70	5.23	28.50	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.936462	0.029611	0.022775	0.011151	93.30	3.99	2.46	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744236	M	M	NA	Agree	0.01	3.15	0.00	20.96	6.39	32.58	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.939206	0.026711	0.02315	0.010932	94.68	3.53	1.56	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744237	M	M	NA	Agree	0.02	3.39	0.00	22.60	6.66	36.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.918922	0.044069	0.024821	0.012189	94.47	3.59	1.77	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744238	M	M	NA	Agree	0.01	3.36	0.00	21.96	6.09	34.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.917411	0.044823	0.024813	0.012953	94.79	3.28	1.76	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744239	M	M	NA	Agree	0.01	3.78	0.00	26.14	5.81	39.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.927827	0.037183	0.021581	0.013409	95.28	2.83	1.72	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744240	M	M	NA	Agree	0.02	4.41	0.00	24.44	6.45	42.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.927124	0.036005	0.024679	0.012192	95.20	3.05	1.59	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744241	M	M	NA	Agree	0.00	3.91	0.00	24.25	6.12	37.86	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.937249	0.029494	0.02159	0.011667	95.37	2.75	1.71	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744242	M	M	NA	Agree	0.00	3.66	0.00	24.54	6.15	41.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.939749	0.027096	0.021445	0.011709	95.48	2.69	1.67	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744243	M	M	NA	Agree	0.01	3.48	0.00	22.28	5.84	38.07	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.935235	0.029673	0.023521	0.011571	94.97	2.92	1.93	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744244	M	M	NA	Agree	0.00	3.06	0.00	21.29	5.35	35.40	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.937685	0.030273	0.021141	0.010901	95.27	2.69	1.86	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744245	M	M	NA	Agree	0.02	3.17	0.00	18.84	5.40	32.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.938005	0.030786	0.023106	0.008104	94.52	3.47	1.82	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does