Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6671274	M	M	NA	Agree	0.00	4.11	0.00	19.65	11.27	66.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884959	0.068113	0.042486	0.004443	93.91	2.64	3.18	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6671275	M	M	NA	Agree	0.00	3.55	0.00	17.61	8.55	69.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886775	0.060204	0.047164	0.005858	91.53	3.14	4.98	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6671276	M	M	NA	Agree	0.00	4.62	0.00	18.37	13.34	74.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.872659	0.076171	0.047007	0.004163	91.88	2.71	5.09	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6671277	M	M	NA	Agree	0.00	3.04	0.00	12.19	13.55	57.28	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.888112	0.074016	0.034291	0.00358	94.41	2.34	3.04	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6671278	M	M	NA	Agree	0.04	2.70	0.00	11.32	12.30	45.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864264	0.063528	0.033038	0.03917	90.82	5.70	3.25	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6671279	M	M	NA	Agree	0.01	3.72	0.00	16.97	17.50	57.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.88198	0.079241	0.037405	0.001374	93.69	2.32	3.73	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6671280	M	M	NA	Agree	0.01	2.33	0.00	11.41	10.07	37.73	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.899801	0.057283	0.039265	0.003651	91.85	2.65	5.20	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6671281	M	M	NA	Agree	0.00	1.48	0.00	7.70	7.53	26.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.927297	0.035406	0.034682	0.002614	93.09	2.44	4.25	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6671282	M	M	NA	Agree	0.01	2.29	0.00	12.62	10.26	38.03	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.900863	0.057901	0.038603	0.002633	92.47	2.59	4.67	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6671283	M	M	NA	Agree	0.03	2.87	0.00	10.91	15.23	47.73	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89552	0.065124	0.037778	0.001578	93.47	2.30	3.96	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6671284	M	M	NA	Agree	0.00	2.72	0.00	10.24	13.55	38.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.900414	0.060998	0.036284	0.002305	93.85	2.36	3.54	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6671285	M	M	NA	Agree	0.03	3.62	0.00	14.02	16.83	55.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889291	0.069384	0.039526	0.001799	94.66	2.26	2.82	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does