Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6665158	M	M	NA	Agree	0.03	8.53	0.00	16.18	9.52	28.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.645615	0.305384	0.046785	0.002217	94.24	3.44	2.03	259	LP2 (2026-09-30)	PASS	NonPolyA_perM 259 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665159	M	M	NA	Agree	0.09	8.13	0.00	18.08	7.26	29.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.643973	0.305058	0.049004	0.001965	94.19	3.47	2.04	331	LP2 (2026-09-30)	PASS	NonPolyA_perM 331 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665160	M	M	NA	Agree	0.02	5.82	0.00	13.45	7.79	21.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.643818	0.307833	0.046289	0.002059	94.05	3.55	2.07	314	LP2 (2026-09-30)	PASS	NonPolyA_perM 314 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665161	M	M	NA	Agree	0.05	8.06	0.00	16.00	7.90	26.90	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.609809	0.340175	0.047728	0.002289	94.15	3.41	2.13	288	LP2 (2026-09-30)	PASS	NonPolyA_perM 288 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665162	M	M	NA	Agree	0.01	5.82	0.00	7.71	3.93	9.95	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.691998	0.24462	0.058681	0.0047	92.00	5.80	1.90	415	LP2 (2026-09-30)	PASS	NonPolyA_perM 415 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665163	M	M	NA	Agree	0.03	7.95	0.00	17.32	7.57	33.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.611449	0.339841	0.045627	0.003083	94.27	3.37	2.05	285	LP2 (2026-09-30)	PASS	NonPolyA_perM 285 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665164	F	F	NA	Agree	29.70	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.645391	0.272092	0.075573	0.006945	89.51	7.82	2.30	758	LP2 (2026-09-30)	PASS	NonPolyA_perM 758 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665165	F	F	NA	Agree	97.98	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.607309	0.343882	0.046343	0.002466	94.76	3.13	1.79	329	LP2 (2026-09-30)	PASS	NonPolyA_perM 329 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665166	F	F	NA	Agree	46.10	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.594679	0.338419	0.063037	0.003866	92.24	5.62	1.82	664	LP2 (2026-09-30)	PASS	NonPolyA_perM 664 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665167	F	F	NA	Agree	75.12	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.597746	0.344094	0.054305	0.003855	92.87	4.26	2.51	582	LP2 (2026-09-30)	PASS	NonPolyA_perM 582 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665168	F	F	NA	Agree	64.56	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.582469	0.355461	0.058636	0.003434	92.78	4.72	2.14	542	LP2 (2026-09-30)	PASS	NonPolyA_perM 542 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665169	F	F	NA	Agree	82.21	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.58666	0.35702	0.05327	0.00305	93.53	3.85	2.26	474	LP2 (2026-09-30)	PASS	NonPolyA_perM 474 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665170	F	F	NA	Agree	78.73	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.627908	0.324242	0.0455	0.00235	94.40	3.41	1.89	319	LP2 (2026-09-30)	PASS	NonPolyA_perM 319 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665171	F	F	NA	Agree	67.40	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.605634	0.337861	0.052492	0.004014	92.96	4.37	2.34	555	LP2 (2026-09-30)	PASS	NonPolyA_perM 555 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665172	M	M	NA	Agree	0.01	6.58	0.00	15.76	5.68	24.95	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.671623	0.278954	0.046846	0.002577	94.26	3.59	1.84	287	LP2 (2026-09-30)	PASS	NonPolyA_perM 287 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665173	M	M	NA	Agree	0.01	5.82	0.00	16.76	5.49	26.78	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.628042	0.322523	0.047281	0.002154	94.30	3.43	1.97	336	LP2 (2026-09-30)	PASS	NonPolyA_perM 336 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665174	M	M	NA	Agree	0.00	5.21	0.00	16.22	6.08	21.11	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.667598	0.271207	0.054247	0.006948	91.89	4.99	2.80	468	LP2 (2026-09-30)	PASS	NonPolyA_perM 468 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665175	M	M	NA	Agree	0.09	5.53	0.00	19.80	6.90	21.95	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.636786	0.299289	0.055423	0.008502	91.39	5.22	3.02	642	LP2 (2026-09-30)	PASS	NonPolyA_perM 642 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665176	F	F	NA	Agree	74.57	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.603486	0.340903	0.05238	0.003231	93.46	4.20	2.02	438	LP2 (2026-09-30)	PASS	NonPolyA_perM 438 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665177	F	F	NA	Agree	69.03	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.607592	0.334058	0.052496	0.005854	92.98	4.34	2.34	533	LP2 (2026-09-30)	PASS	NonPolyA_perM 533 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665178	F	F	NA	Agree	75.50	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.618642	0.323482	0.054575	0.003302	93.14	4.11	2.43	478	LP2 (2026-09-30)	PASS	NonPolyA_perM 478 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665179	F	F	NA	Agree	79.54	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.595371	0.347009	0.054181	0.003439	93.43	3.90	2.35	487	LP2 (2026-09-30)	PASS	NonPolyA_perM 487 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665180	F	F	NA	Agree	86.66	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.608245	0.335635	0.053658	0.002461	93.10	4.10	2.47	471	LP2 (2026-09-30)	PASS	NonPolyA_perM 471 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665181	F	F	NA	Agree	90.99	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.608617	0.341222	0.048113	0.002048	93.54	3.26	2.88	466	LP2 (2026-09-30)	PASS	NonPolyA_perM 466 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665182	F	F	NA	Agree	123.36	0.03	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.745863	0.218236	0.033138	0.002763	94.20	2.95	2.61	248	LP2 (2026-09-30)	PASS	NonPolyA_perM 248 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665183	F	F	NA	Agree	111.73	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.624086	0.322956	0.04858	0.004379	93.16	4.09	2.41	488	LP2 (2026-09-30)	PASS	NonPolyA_perM 488 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665184	M	M	NA	Agree	0.01	4.90	0.00	12.53	5.60	21.91	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.655075	0.292203	0.047785	0.004937	93.40	4.00	2.31	357	LP2 (2026-09-30)	PASS	NonPolyA_perM 357 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665185	M	M	NA	Agree	0.01	6.73	0.00	17.45	6.57	29.55	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.606482	0.344794	0.04705	0.001674	94.29	3.20	2.20	375	LP2 (2026-09-30)	PASS	NonPolyA_perM 375 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665186	M	M	NA	Agree	0.01	0.94	0.00	0.83	1.09	1.88	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.593589	0.327291	0.074759	0.004361	90.51	7.15	2.07	928	LP2 (2026-09-30)	PASS	NonPolyA_perM 928 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665187	M	M	NA	Agree	0.03	10.68	0.00	20.02	10.39	33.29	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.590378	0.36007	0.047962	0.00159	94.67	2.99	2.04	343	LP2 (2026-09-30)	PASS	NonPolyA_perM 343 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665188	M	M	NA	Agree	0.01	7.82	0.00	15.73	5.44	25.91	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.690388	0.258557	0.048672	0.002382	94.01	3.69	1.98	276	LP2 (2026-09-30)	PASS	NonPolyA_perM 276 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665189	M	M	NA	Agree	0.06	8.95	0.00	17.09	7.92	28.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.653065	0.294138	0.048796	0.004001	93.29	3.58	2.83	265	LP2 (2026-09-30)	PASS	NonPolyA_perM 265 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665190	M	M	NA	Agree	0.02	5.77	0.00	10.96	5.71	18.39	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.68163	0.267254	0.048792	0.002324	94.03	3.92	1.75	302	LP2 (2026-09-30)	PASS	NonPolyA_perM 302 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665191	M	M	NA	Agree	0.03	7.85	0.00	16.57	7.17	30.90	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.639857	0.311035	0.047212	0.001897	94.22	3.29	2.19	271	LP2 (2026-09-30)	PASS	NonPolyA_perM 271 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665192	F	F	NA	Agree	88.79	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.615882	0.336463	0.045642	0.002014	94.17	3.30	2.22	324	LP2 (2026-09-30)	PASS	NonPolyA_perM 324 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665193	F	F	NA	Agree	81.30	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.6291	0.322861	0.045918	0.002121	94.60	3.20	1.90	314	LP2 (2026-09-30)	PASS	NonPolyA_perM 314 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665194	F	F	NA	Agree	73.14	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.613245	0.319359	0.049613	0.017783	92.35	5.22	2.12	496	LP2 (2026-09-30)	PASS	NonPolyA_perM 496 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665195	F	F	NA	Agree	77.34	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.62441	0.316563	0.051755	0.007272	92.95	4.36	2.37	465	LP2 (2026-09-30)	PASS	NonPolyA_perM 465 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665196	M	Undetermined	NA	Conflict	0.00	0.63	0.00	0.89	0.92	2.49	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.632099	0.265159	0.096192	0.00655	87.26	9.97	2.45	1343	LP2 (2026-09-30)	PASS	NonPolyA_perM 1343 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665197	M	M	NA	Agree	0.06	6.09	0.00	16.67	5.36	26.08	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.570256	0.371093	0.053005	0.005646	93.15	3.94	2.59	559	LP2 (2026-09-30)	PASS	NonPolyA_perM 559 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665198	M	Undetermined	NA	Conflict	0.00	0.85	0.00	0.75	0.81	1.86	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.709931	0.194386	0.088264	0.007419	87.53	10.02	2.11	883	LP2 (2026-09-30)	PASS	NonPolyA_perM 883 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665199	M	M	NA	Agree	0.04	5.56	0.00	16.27	4.98	24.44	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.578843	0.366193	0.052744	0.00222	93.05	3.73	2.89	612	LP2 (2026-09-30)	PASS	NonPolyA_perM 612 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665200	M	M	NA	Agree	0.00	0.58	0.00	1.17	0.84	1.85	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.685274	0.151299	0.150539	0.012887	79.23	17.35	3.09	2449	LP2 (2026-09-30)	PASS	NonPolyA_perM 2449 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665201	M	M	NA	Agree	0.01	6.59	0.00	17.77	7.33	30.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.576649	0.373824	0.048116	0.001411	94.74	2.89	2.06	346	LP2 (2026-09-30)	PASS	NonPolyA_perM 346 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665202	M	M	NA	Agree	0.00	0.79	0.00	2.25	0.95	2.71	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.621663	0.252582	0.116911	0.008845	83.34	12.68	3.64	2135	LP2 (2026-09-30)	PASS	NonPolyA_perM 2135 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665203	M	M	NA	Agree	0.05	9.44	0.00	20.92	8.70	39.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.616934	0.333423	0.048251	0.001392	94.77	2.91	2.01	219	LP2 (2026-09-30)	PASS	NonPolyA_perM 219 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665204	F	F	NA	Agree	44.15	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.525053	0.382129	0.085551	0.007267	87.91	8.17	3.57	1208	LP2 (2026-09-30)	PASS	NonPolyA_perM 1208 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665205	F	F	NA	Agree	80.93	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.586533	0.358971	0.051198	0.003298	93.18	3.65	2.85	502	LP2 (2026-09-30)	PASS	NonPolyA_perM 502 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665206	F	F	NA	Agree	30.74	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.629658	0.288778	0.076643	0.004921	89.24	7.93	2.44	906	LP2 (2026-09-30)	PASS	NonPolyA_perM 906 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665207	F	F	NA	Agree	115.44	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.579951	0.373353	0.045618	0.001077	94.83	2.61	2.21	323	LP2 (2026-09-30)	PASS	NonPolyA_perM 323 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665208	F	F	NA	Agree	19.96	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.57744	0.2898	0.121856	0.010904	81.36	13.57	4.52	1838	LP2 (2026-09-30)	PASS	NonPolyA_perM 1838 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665209	F	F	NA	Agree	100.75	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.606386	0.346251	0.045726	0.001637	94.47	3.08	2.11	331	LP2 (2026-09-30)	PASS	NonPolyA_perM 331 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665210	F	F	NA	Agree	45.10	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.574942	0.348052	0.07153	0.005476	90.71	6.61	2.36	656	LP2 (2026-09-30)	PASS	NonPolyA_perM 656 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665211	F	F	NA	Agree	100.60	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.603376	0.347624	0.046735	0.002265	94.27	3.19	2.21	307	LP2 (2026-09-30)	PASS	NonPolyA_perM 307 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665212	F	F	NA	Agree	40.45	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.562182	0.354577	0.078484	0.004757	89.91	7.45	2.30	929	LP2 (2026-09-30)	PASS	NonPolyA_perM 929 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665213	F	F	NA	Agree	121.59	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.594722	0.353183	0.050528	0.001568	94.39	3.01	2.27	335	LP2 (2026-09-30)	PASS	NonPolyA_perM 335 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665214	M	Undetermined	NA	Conflict	0.00	0.39	0.00	0.85	0.74	1.82	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.701226	0.120356	0.15688	0.021537	76.04	19.43	4.23	3021	LP2 (2026-09-30)	PASS	NonPolyA_perM 3021 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665215	M	Undetermined	NA	Conflict	49.26	3.42	0.00	12.03	3.18	15.70	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.580517	0.36933	0.047721	0.002432	93.86	3.12	2.70	386	LP2 (2026-09-30)	PASS	NonPolyA_perM 386 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665216	M	M	NA	Agree	0.00	0.67	0.00	1.27	1.11	2.21	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.737647	0.140693	0.112546	0.009115	83.78	12.72	3.20	1523	LP2 (2026-09-30)	PASS	NonPolyA_perM 1523 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665217	M	M	NA	Agree	0.01	8.20	0.00	19.85	6.78	33.20	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.583249	0.363872	0.051123	0.001757	93.98	3.14	2.56	407	LP2 (2026-09-30)	PASS	NonPolyA_perM 407 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665218	M	M	NA	Agree	0.00	0.82	0.00	1.01	1.22	2.26	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.636951	0.260891	0.092325	0.009833	86.90	10.08	2.66	1172	LP2 (2026-09-30)	PASS	NonPolyA_perM 1172 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665219	M	M	NA	Agree	0.03	6.80	0.00	16.66	6.29	31.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.628085	0.321426	0.048404	0.002086	94.74	3.18	1.77	302	LP2 (2026-09-30)	PASS	NonPolyA_perM 302 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665220	M	Undetermined	NA	Conflict	0.00	0.52	0.00	0.63	0.88	1.80	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.740833	0.149495	0.101009	0.008664	86.29	11.42	1.95	1024	LP2 (2026-09-30)	PASS	NonPolyA_perM 1024 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665221	M	M	NA	Agree	0.01	5.33	0.00	14.47	5.80	26.84	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.619121	0.321778	0.055867	0.003234	92.62	4.13	2.92	480	LP2 (2026-09-30)	PASS	NonPolyA_perM 480 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665222	F	F	NA	Agree	54.54	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.608314	0.333294	0.055339	0.003054	92.86	4.64	2.21	453	LP2 (2026-09-30)	PASS	NonPolyA_perM 453 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665223	F	F	NA	Agree	92.98	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.586537	0.358429	0.048376	0.006658	93.29	3.87	2.50	493	LP2 (2026-09-30)	PASS	NonPolyA_perM 493 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665224	F	F	NA	Agree	52.51	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.559455	0.340125	0.093488	0.006932	88.30	9.07	2.29	811	LP2 (2026-09-30)	PASS	NonPolyA_perM 811 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665225	F	F	NA	Agree	69.72	2.32	0.00	5.44	2.52	10.52	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.592365	0.353089	0.052464	0.002082	94.05	3.53	2.08	375	LP2 (2026-09-30)	PASS	NonPolyA_perM 375 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665226	F	F	NA	Agree	60.82	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.569748	0.327741	0.094133	0.008377	88.21	9.26	2.23	631	LP2 (2026-09-30)	PASS	NonPolyA_perM 631 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665227	F	F	NA	Agree	87.53	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.598314	0.337162	0.059681	0.004844	92.84	4.55	2.27	460	LP2 (2026-09-30)	PASS	NonPolyA_perM 460 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665228	M	M	NA	Agree	0.01	0.87	0.00	1.77	1.53	3.36	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.584521	0.315476	0.087096	0.012906	87.54	9.10	3.03	1119	LP2 (2026-09-30)	PASS	NonPolyA_perM 1119 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665229	M	M	NA	Agree	0.00	5.89	0.00	13.71	5.06	22.77	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.669492	0.275393	0.050427	0.004688	92.09	4.02	3.61	469	LP2 (2026-09-30)	PASS	NonPolyA_perM 469 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665230	M	M	NA	Agree	0.00	1.06	0.00	1.49	1.16	3.01	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.622634	0.291731	0.079618	0.006018	89.26	8.29	2.11	878	LP2 (2026-09-30)	PASS	NonPolyA_perM 878 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665231	M	M	NA	Agree	0.00	7.55	0.00	17.63	8.70	39.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.597217	0.339528	0.04858	0.014675	93.04	4.36	2.28	265	LP2 (2026-09-30)	PASS	NonPolyA_perM 265 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665232	M	M	NA	Agree	0.00	1.32	0.00	1.06	1.38	4.75	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.658303	0.267672	0.070756	0.003269	89.82	7.43	2.37	720	LP2 (2026-09-30)	PASS	NonPolyA_perM 720 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665233	M	M	NA	Agree	0.00	6.19	0.00	12.06	7.43	27.27	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.552074	0.396007	0.05042	0.0015	94.16	3.20	2.31	424	LP2 (2026-09-30)	PASS	NonPolyA_perM 424 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665234	M	Undetermined	NA	Conflict	0.00	0.52	0.00	1.29	0.57	1.45	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.637681	0.217467	0.132308	0.012543	81.11	15.31	3.24	2260	LP2 (2026-09-30)	PASS	NonPolyA_perM 2260 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665235	M	M	NA	Agree	0.01	4.50	0.00	10.47	6.67	25.92	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.661221	0.284733	0.051727	0.00232	93.73	3.72	2.25	284	LP2 (2026-09-30)	PASS	NonPolyA_perM 284 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6665236	F	F	NA	Agree	63.53	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.655103	0.283054	0.056447	0.005397	92.26	4.96	2.46	419	LP2 (2026-09-30)	PASS	NonPolyA_perM 419 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665237	F	F	NA	Agree	80.65	0.04	0.00	0.02	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.650768	0.294393	0.047829	0.007011	93.68	3.71	2.30	363	LP2 (2026-09-30)	PASS	NonPolyA_perM 363 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM6665238	F	F	NA	Agree	43.20	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.581516	0.327341	0.084446	0.006697	89.21	7.80	2.60	911	LP2 (2026-09-30)	PASS	NonPolyA_perM 911 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM6665239	F	F	NA	Agree	129.67	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.579929	0.370934	0.047342	0.001795	94.87	2.76	2.05	323	LP2 (2026-09-30)	PASS	NonPolyA_perM 323 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does