Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-16) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6547517	M	M	NA	Agree	0.01	1.85	0.00	14.12	3.66	23.54	NONE	POLYA	0.891471	0.03246	0.018858	0.057211	88.44	8.07	3.03	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547518	M	M	NA	Agree	0.04	1.81	0.00	16.61	3.36	24.27	NONE	POLYA	0.89823	0.03247	0.018214	0.051086	89.51	7.15	2.82	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547519	M	M	NA	Agree	0.05	1.89	0.00	15.88	3.83	23.58	NONE	POLYA	0.907199	0.034693	0.016989	0.041119	90.33	6.33	2.92	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547520	M	M	NA	Agree	0.02	1.68	0.00	14.31	3.89	20.14	NONE	POLYA	0.822761	0.035463	0.016074	0.125702	82.35	14.47	2.76	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547521	M	M	NA	Agree	0.02	1.46	0.00	14.31	3.82	20.42	NONE	POLYA	0.915615	0.031947	0.01722	0.035218	91.66	5.71	2.21	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547522	M	M	NA	Agree	0.01	1.71	0.00	11.39	2.72	24.14	NONE	POLYA	0.88464	0.027716	0.018575	0.069069	87.19	9.17	3.22	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547523	M	M	NA	Agree	0.00	2.04	0.00	16.59	2.35	25.10	NONE	POLYA	0.908432	0.02888	0.017258	0.04543	90.01	6.56	3.06	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547524	M	M	NA	Agree	0.04	2.79	0.00	15.87	3.41	25.15	NONE	POLYA	0.895453	0.036288	0.017621	0.050638	89.15	7.56	2.70	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547525	M	M	NA	Agree	0.01	2.30	0.00	11.86	3.15	24.68	NONE	POLYA	0.906742	0.022207	0.019632	0.051419	89.00	7.26	3.28	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547526	M	M	NA	Agree	0.01	2.53	0.00	13.80	3.71	28.64	NONE	POLYA	0.910246	0.027595	0.01909	0.04307	89.90	6.35	3.38	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547527	M	M	NA	Agree	0.03	1.54	0.00	16.21	1.76	18.85	NONE	POLYA	0.865747	0.037419	0.020454	0.076379	87.03	9.70	2.95	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547528	M	M	NA	Agree	0.04	1.45	0.00	12.64	3.83	20.88	NONE	POLYA	0.817695	0.039153	0.021441	0.12171	82.40	14.13	2.95	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547529	M	M	NA	Agree	0.02	1.44	0.00	14.36	1.26	18.26	NONE	POLYA	0.910342	0.029551	0.019867	0.04024	90.47	6.26	2.90	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547530	M	M	NA	Agree	0.01	1.60	0.00	13.86	2.41	22.55	NONE	POLYA	0.831005	0.037201	0.021589	0.110205	83.96	13.02	2.54	150	LP2 (2026-09-30)	PASS	NonPolyA_perM 150 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547531	M	M	NA	Agree	0.00	1.95	0.00	15.57	3.59	22.26	NONE	POLYA	0.891532	0.036445	0.020662	0.051362	89.83	7.52	2.25	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547532	M	M	NA	Agree	0.01	2.11	0.00	16.09	4.18	22.04	NONE	POLYA	0.874995	0.041352	0.023087	0.060566	88.83	8.12	2.60	170	LP2 (2026-09-30)	PASS	NonPolyA_perM 170 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547533	M	M	NA	Agree	0.02	3.00	0.00	23.25	2.87	25.97	NONE	POLYA	0.929009	0.030579	0.021974	0.018438	81.67	3.34	14.71	162	LP2 (2026-09-30)	PASS	NonPolyA_perM 162 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547534	M	M	NA	Agree	0.03	2.11	0.00	16.80	3.22	28.23	NONE	POLYA	0.87959	0.027674	0.019583	0.073153	87.15	9.11	3.41	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547535	M	M	NA	Agree	0.02	1.86	0.00	15.68	2.09	19.16	NONE	POLYA	0.869576	0.024868	0.018695	0.086862	86.58	10.19	2.85	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547536	M	M	NA	Agree	0.04	2.39	0.00	19.45	2.51	20.87	NONE	POLYA	0.903503	0.028638	0.018031	0.049827	88.87	7.21	3.50	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547537	M	M	NA	Agree	0.01	2.35	0.00	14.20	4.06	28.81	NONE	POLYA	0.873128	0.029796	0.02047	0.076607	87.06	9.51	3.07	175	LP2 (2026-09-30)	PASS	NonPolyA_perM 175 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6547538	M	M	NA	Agree	0.02	2.31	0.00	18.59	2.55	21.99	NONE	POLYA	0.820995	0.038418	0.01809	0.122497	82.43	13.97	3.04	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does