Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6429333	not_specified	M	NA	Conflict	0.02	1.58	0.00	9.24	2.87	29.02	NONE	POLYA	0.913992	0.051719	0.023634	0.010654	92.86	3.19	3.19	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6429334	not_specified	M	NA	Conflict	0.00	1.37	0.00	11.10	3.61	33.69	NONE	POLYA	0.917093	0.040844	0.022538	0.019525	92.81	3.75	3.11	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6429335	not_specified	M	NA	Conflict	0.01	1.31	0.00	12.41	3.94	28.00	NONE	POLYA	0.916918	0.047635	0.023318	0.012129	93.31	2.95	3.43	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6429336	not_specified	M	NA	Conflict	0.00	1.22	0.00	9.31	3.09	31.24	NONE	POLYA	0.923753	0.03894	0.023177	0.014129	92.97	3.30	3.45	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6429337	not_specified	M	NA	Conflict	0.01	2.13	0.00	13.12	3.54	29.88	NONE	POLYA	0.882549	0.052468	0.023966	0.041017	90.58	5.74	3.32	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6429338	not_specified	M	NA	Conflict	0.00	0.68	0.00	6.84	2.71	29.52	NONE	POLYA	0.893209	0.038769	0.020024	0.047998	89.51	6.31	3.91	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6429339	not_specified	M	NA	Conflict	0.00	1.14	0.00	4.86	2.12	27.60	NONE	POLYA	0.916598	0.037634	0.019976	0.025792	91.67	4.46	3.30	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6429340	not_specified	M	NA	Conflict	0.00	1.37	0.00	7.68	3.79	29.39	NONE	POLYA	0.901025	0.054478	0.020276	0.024221	91.85	4.28	3.29	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6429341	not_specified	M	NA	Conflict	0.00	0.86	0.00	5.05	2.77	26.24	NONE	POLYA	0.921963	0.032695	0.01875	0.026592	91.96	4.44	3.09	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6429342	not_specified	M	NA	Conflict	0.01	1.03	0.00	6.09	2.47	27.07	NONE	POLYA	0.902291	0.035964	0.019304	0.04244	90.25	5.96	3.10	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6429343	not_specified	M	NA	Conflict	0.01	0.91	0.00	7.62	2.42	27.47	NONE	POLYA	0.905686	0.034007	0.019931	0.040376	90.14	5.79	3.46	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6429344	not_specified	M	NA	Conflict	0.01	0.52	0.00	7.29	3.00	25.54	NONE	POLYA	0.921603	0.03853	0.019934	0.019933	92.01	3.86	3.50	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does