Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6412387	M	M	NA	Agree	0.00	5.85	0.00	33.16	22.24	56.90	NONE	POLYA	0.813653	0.123424	0.04815	0.014774	94.36	2.60	2.87	131	LP2 (2026-09-30)	PASS	NonPolyA_perM 131 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412388	M	M	NA	Agree	0.00	7.11	0.00	35.37	20.41	60.79	NONE	POLYA	0.827184	0.110994	0.046684	0.015137	95.04	2.68	2.11	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412389	M	M	NA	Agree	0.00	8.12	0.00	34.65	18.07	60.35	NONE	POLYA	0.8128	0.119629	0.046066	0.021505	94.13	3.22	2.48	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412390	M	M	NA	Agree	0.06	7.48	0.00	37.30	20.52	61.79	NONE	POLYA	0.800905	0.129794	0.046656	0.022645	93.85	3.44	2.52	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412391	M	M	NA	Agree	0.00	6.64	0.00	38.98	23.98	61.67	NONE	POLYA	0.818564	0.120478	0.0479	0.013058	95.22	2.45	2.14	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412392	M	M	NA	Agree	0.00	7.07	0.00	37.70	22.21	62.16	NONE	POLYA	0.8155	0.12125	0.047275	0.015975	94.94	2.66	2.21	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412393	M	M	NA	Agree	0.01	6.30	0.00	27.14	20.62	70.58	NONE	POLYA	0.829774	0.106264	0.047451	0.016511	94.86	2.75	2.20	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412394	M	M	NA	Agree	0.02	6.43	0.00	34.61	21.19	66.07	NONE	POLYA	0.818405	0.119454	0.047793	0.014348	95.03	2.53	2.25	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412395	M	M	NA	Agree	0.00	7.08	0.00	41.09	24.52	57.26	NONE	POLYA	0.80128	0.132308	0.047827	0.018585	94.41	2.86	2.53	130	LP2 (2026-09-30)	PASS	NonPolyA_perM 130 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412396	M	M	NA	Agree	0.00	7.01	0.00	36.65	23.08	57.85	NONE	POLYA	0.801104	0.133332	0.047567	0.017997	94.76	2.85	2.19	135	LP2 (2026-09-30)	PASS	NonPolyA_perM 135 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412397	M	M	NA	Agree	0.01	6.96	0.00	40.01	18.12	59.33	NONE	POLYA	0.797363	0.13491	0.047875	0.019851	94.38	3.13	2.28	139	LP2 (2026-09-30)	PASS	NonPolyA_perM 139 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412398	M	M	NA	Agree	0.00	6.90	0.00	36.64	16.06	53.48	NONE	POLYA	0.799036	0.130054	0.047116	0.023795	93.62	3.55	2.62	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412399	M	M	NA	Agree	0.00	8.12	0.00	36.79	20.46	57.43	NONE	POLYA	0.800168	0.131988	0.046834	0.021011	93.82	3.25	2.73	137	LP2 (2026-09-30)	PASS	NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412400	M	M	NA	Agree	0.08	6.16	0.00	36.76	17.94	58.78	NONE	POLYA	0.787825	0.141124	0.049038	0.022014	94.08	3.31	2.38	142	LP2 (2026-09-30)	PASS	NonPolyA_perM 142 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412401	M	M	NA	Agree	0.03	5.66	0.00	34.05	18.06	50.50	NONE	POLYA	0.776165	0.148668	0.046282	0.028885	93.36	3.97	2.46	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412402	M	M	NA	Agree	0.01	6.97	0.00	36.89	16.80	63.16	NONE	POLYA	0.778548	0.144135	0.047482	0.029835	93.19	3.92	2.67	129	LP2 (2026-09-30)	PASS	NonPolyA_perM 129 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412403	M	M	NA	Agree	0.00	8.07	0.00	38.10	19.36	60.96	NONE	POLYA	0.800517	0.131485	0.048991	0.019007	94.50	3.01	2.28	136	LP2 (2026-09-30)	PASS	NonPolyA_perM 136 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412404	M	M	NA	Agree	0.00	7.13	0.00	41.09	21.50	66.10	NONE	POLYA	0.789077	0.140418	0.049454	0.021051	93.87	3.22	2.71	152	LP2 (2026-09-30)	PASS	NonPolyA_perM 152 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412405	M	M	NA	Agree	0.00	7.44	0.00	36.72	17.86	58.60	NONE	POLYA	0.776379	0.152554	0.047917	0.02315	93.50	3.39	2.88	147	LP2 (2026-09-30)	PASS	NonPolyA_perM 147 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412406	M	M	NA	Agree	0.00	3.60	0.00	29.18	14.37	38.45	NONE	POLYA	0.823752	0.108151	0.04478	0.023317	93.98	3.41	2.43	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412407	M	M	NA	Agree	0.02	7.69	0.00	43.69	18.84	50.94	NONE	POLYA	0.822565	0.1205	0.048	0.008935	95.55	2.70	1.51	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412408	M	M	NA	Agree	0.08	7.99	0.00	29.06	14.96	51.18	NONE	POLYA	0.831639	0.110469	0.044501	0.013391	95.01	2.58	2.24	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412409	M	M	NA	Agree	0.04	6.24	0.00	30.12	14.86	51.83	NONE	POLYA	0.83114	0.110269	0.045833	0.012758	95.19	2.50	2.12	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412410	M	M	NA	Agree	0.13	6.86	0.00	32.47	14.05	52.30	NONE	POLYA	0.825993	0.11531	0.044882	0.013814	94.34	2.51	2.97	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412411	M	M	NA	Agree	0.08	5.74	0.00	27.94	11.26	48.37	NONE	POLYA	0.822476	0.108589	0.043989	0.024947	93.83	3.53	2.46	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412412	M	M	NA	Agree	0.05	5.57	0.00	29.03	15.21	53.91	NONE	POLYA	0.808733	0.112477	0.046629	0.032161	93.38	4.94	1.46	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412413	M	M	NA	Agree	0.14	6.50	0.00	37.77	15.72	53.13	NONE	POLYA	0.811963	0.119941	0.04642	0.021677	94.34	3.94	1.47	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412414	M	M	NA	Agree	0.01	10.41	0.00	51.02	25.63	65.66	NONE	POLYA	0.669586	0.214901	0.049207	0.066307	90.29	6.89	2.55	218	LP2 (2026-09-30)	PASS	NonPolyA_perM 218 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412415	M	M	NA	Agree	0.00	13.05	0.00	52.69	28.38	62.11	NONE	POLYA	0.777835	0.161979	0.050863	0.009323	95.42	1.97	2.41	165	LP2 (2026-09-30)	PASS	NonPolyA_perM 165 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412416	M	M	NA	Agree	0.00	11.81	0.00	53.69	24.69	64.28	NONE	POLYA	0.744734	0.187053	0.05168	0.016533	94.51	2.67	2.57	195	LP2 (2026-09-30)	PASS	NonPolyA_perM 195 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412417	M	M	NA	Agree	0.02	13.41	0.00	55.65	26.90	69.68	NONE	POLYA	0.769278	0.167354	0.051608	0.01176	95.25	2.22	2.31	173	LP2 (2026-09-30)	PASS	NonPolyA_perM 173 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412418	M	M	NA	Agree	0.00	11.59	0.00	49.11	25.00	69.11	NONE	POLYA	0.784731	0.154073	0.049807	0.011389	94.75	2.23	2.80	137	LP2 (2026-09-30)	PASS	NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412419	M	M	NA	Agree	0.00	10.00	0.00	48.72	25.87	64.84	NONE	POLYA	0.789709	0.147153	0.050483	0.012655	95.16	2.26	2.37	157	LP2 (2026-09-30)	PASS	NonPolyA_perM 157 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412420	M	M	NA	Agree	0.00	11.18	0.00	51.05	23.78	73.10	NONE	POLYA	0.779775	0.157612	0.050353	0.012259	95.22	2.28	2.29	169	LP2 (2026-09-30)	PASS	NonPolyA_perM 169 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412421	M	M	NA	Agree	0.00	11.94	0.00	51.57	26.73	73.59	NONE	POLYA	0.769946	0.165017	0.051424	0.013613	95.24	2.38	2.18	191	LP2 (2026-09-30)	PASS	NonPolyA_perM 191 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6412422	M	M	NA	Agree	0.00	11.51	0.00	47.32	25.57	62.96	NONE	POLYA	0.787293	0.153937	0.050518	0.008252	95.48	1.93	2.37	160	LP2 (2026-09-30)	PASS	NonPolyA_perM 160 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does