Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6257362	M	F	NA	Conflict	155.24	0.00	0.00	0.04	0.00	0.00	NONE	POLYA	0.889496	0.077894	0.032498	0.000111	93.59	2.26	3.82	135	LP2 (2026-09-30)	PASS	NonPolyA_perM 135 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6257363	M	F	NA	Conflict	152.10	0.00	0.00	0.00	0.00	0.05	NONE	POLYA	0.894349	0.072603	0.032921	0.000128	93.55	2.15	4.04	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6257364	M	F	NA	Conflict	152.67	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.8923	0.074252	0.033268	0.00018	93.54	2.21	3.96	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6257365	M	M	NA	Agree	0.11	8.65	0.00	31.97	13.70	59.78	NONE	POLYA	0.894999	0.07015	0.034759	0.000092	93.58	2.21	3.87	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6257366	M	M	NA	Agree	0.11	6.40	0.00	31.88	11.99	40.60	NONE	POLYA	0.895707	0.072111	0.032059	0.000123	93.15	2.48	3.93	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6257367	M	M	NA	Agree	0.18	14.42	0.00	43.31	17.25	49.14	NONE	POLYA	0.869449	0.09324	0.037096	0.000216	91.86	2.58	5.15	144	LP2 (2026-09-30)	PASS	NonPolyA_perM 144 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6257368	M	M	NA	Agree	0.22	15.03	0.00	55.84	21.21	69.30	NONE	POLYA	0.870374	0.090985	0.038547	0.000094	92.75	2.14	4.66	140	LP2 (2026-09-30)	PASS	NonPolyA_perM 140 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6257369	M	M	NA	Agree	0.34	16.54	0.00	49.23	21.03	61.66	NONE	POLYA	0.862398	0.10015	0.03714	0.000312	91.88	2.61	5.01	147	LP2 (2026-09-30)	PASS	NonPolyA_perM 147 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6257370	M	M	NA	Agree	0.25	16.71	0.00	41.63	18.83	59.20	NONE	POLYA	0.884723	0.080294	0.034865	0.000118	92.66	2.54	4.43	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does