Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6243478	M	M	NA	Agree	0.01	1.06	0.00	17.47	4.38	38.11	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.941921	0.034262	0.022273	0.001544	93.20	1.72	4.90	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6243479	M	M	NA	Agree	0.15	2.60	0.00	24.13	10.37	67.50	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.91423	0.052127	0.030776	0.002867	92.33	2.00	5.48	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6243480	M	M	NA	Agree	0.00	1.79	0.00	13.81	7.22	53.30	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.904297	0.051768	0.040184	0.003751	92.16	2.33	5.27	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6243481	M	M	NA	Agree	0.03	1.64	0.00	17.17	6.70	50.36	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.907422	0.05349	0.032981	0.006107	91.74	2.33	5.72	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6243482	M	M	NA	Agree	0.09	1.88	0.00	20.32	5.83	47.30	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.921393	0.047781	0.029041	0.001785	92.84	1.85	5.11	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6243483	M	M	NA	Agree	0.04	2.38	0.00	20.94	9.39	49.38	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.933351	0.035716	0.024464	0.006469	91.92	2.41	5.45	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6243484	M	M	NA	Agree	0.00	1.69	0.00	14.32	5.00	60.60	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.935322	0.028658	0.029006	0.007014	91.99	2.47	5.35	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6243485	M	M	NA	Agree	0.16	1.72	0.00	24.92	8.29	46.49	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.928024	0.044492	0.023384	0.004099	93.20	1.83	4.83	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6243486	M	M	NA	Agree	0.15	2.75	0.00	25.48	9.51	47.18	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.923361	0.046573	0.028133	0.001933	92.42	1.78	5.35	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6243487	M	M	NA	Agree	0.09	2.17	0.00	21.28	8.68	53.04	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.931147	0.041018	0.026592	0.001242	92.70	1.89	5.23	114	LP2 (2026-09-30)	PASS	NonPolyA_perM 114 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6243488	M	M	NA	Agree	0.08	2.03	0.00	22.60	7.24	50.36	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.926772	0.043526	0.028022	0.001681	92.61	1.91	5.31	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6243489	M	Undetermined	NA	Conflict	0.00	0.18	0.00	0.56	0.74	40.17	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.922282	0.028308	0.029908	0.019503	86.68	4.04	9.09	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does