Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6066054	not_specified	M	NA	Conflict	0.01	10.24	0.00	39.20	17.92	51.37	NONE	POLYA	0.922907	0.029214	0.034686	0.013193	91.98	2.78	5.06	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6066055	not_specified	M	NA	Conflict	0.01	8.46	0.00	37.29	17.31	50.55	NONE	POLYA	0.905992	0.036035	0.035326	0.022646	92.13	3.66	4.01	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6066056	not_specified	M	NA	Conflict	0.02	7.14	0.00	32.09	15.66	49.15	NONE	POLYA	0.910387	0.040024	0.036781	0.012808	92.35	2.97	4.49	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6066057	not_specified	M	NA	Conflict	0.02	8.61	0.00	36.36	16.50	51.82	NONE	POLYA	0.93402	0.028987	0.035233	0.00176	96.32	1.78	1.71	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6066058	not_specified	F	NA	Conflict	38.30	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.930316	0.025098	0.034855	0.009731	93.22	2.51	4.08	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6066059	not_specified	M	NA	Conflict	0.02	10.47	0.00	49.66	19.37	58.73	NONE	POLYA	0.901774	0.04892	0.036317	0.012988	92.45	2.62	4.74	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6066060	not_specified	M	NA	Conflict	0.06	10.74	0.00	47.39	18.92	58.00	NONE	POLYA	0.902825	0.053502	0.036213	0.00746	93.42	2.32	4.04	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6066061	not_specified	F	NA	Conflict	69.75	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.915597	0.038055	0.035376	0.010972	93.04	2.76	4.00	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6066062	not_specified	F	NA	Conflict	94.34	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.914642	0.036499	0.034214	0.014645	92.73	2.99	4.10	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6066063	not_specified	M	NA	Conflict	0.00	9.08	0.00	32.23	17.30	53.46	NONE	POLYA	0.925961	0.025947	0.034267	0.013825	92.75	2.90	4.14	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does