Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6037837	M	M	NA	Agree	0.02	3.85	0.00	19.88	11.67	52.20	NONE	POLYA	0.858913	0.089874	0.04663	0.004583	78.26	1.94	19.59	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037838	M	M	NA	Agree	0.01	5.36	0.00	20.84	12.20	58.92	NONE	POLYA	0.85421	0.092145	0.046914	0.006731	82.44	2.24	15.12	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037839	M	M	NA	Agree	0.02	5.45	0.00	25.35	13.37	61.56	NONE	POLYA	0.850481	0.096447	0.046885	0.006187	80.81	2.04	16.95	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037840	M	M	NA	Agree	0.04	4.63	0.00	23.53	11.17	67.86	NONE	POLYA	0.856761	0.089099	0.046669	0.007471	82.40	2.32	15.07	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037841	M	M	NA	Agree	0.01	3.74	0.00	21.23	9.13	53.96	NONE	POLYA	0.862548	0.085079	0.045645	0.006728	79.63	2.21	17.98	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037842	M	M	NA	Agree	0.02	4.39	0.00	20.98	13.22	63.30	NONE	POLYA	0.859881	0.08876	0.047081	0.004278	80.27	1.97	17.55	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037843	M	M	NA	Agree	0.02	5.08	0.00	22.65	12.75	53.28	NONE	POLYA	0.853184	0.093644	0.046606	0.006567	80.27	2.13	17.39	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037844	M	M	NA	Agree	0.00	4.09	0.00	19.80	13.02	53.42	NONE	POLYA	0.855863	0.092371	0.045096	0.006671	78.72	2.11	18.97	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037845	M	M	NA	Agree	0.01	4.48	0.00	23.03	13.72	60.28	NONE	POLYA	0.851355	0.094944	0.046171	0.00753	80.40	2.24	17.16	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037846	M	M	NA	Agree	0.01	4.36	0.00	21.72	11.77	50.93	NONE	POLYA	0.856786	0.091011	0.045386	0.006816	77.40	2.11	20.29	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037847	M	M	NA	Agree	0.02	5.21	0.00	24.06	13.01	56.57	NONE	POLYA	0.850044	0.094051	0.047848	0.008057	79.69	2.28	17.80	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037848	M	M	NA	Agree	0.02	5.10	0.00	23.81	14.24	57.46	NONE	POLYA	0.858404	0.089128	0.046842	0.005626	78.74	2.00	19.05	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037849	M	M	NA	Agree	0.00	6.11	0.00	26.11	13.04	59.48	NONE	POLYA	0.846225	0.098891	0.051403	0.003481	79.83	1.82	18.13	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037850	M	M	NA	Agree	0.02	5.58	0.00	29.21	14.14	62.17	NONE	POLYA	0.849648	0.097345	0.049469	0.003538	80.52	1.75	17.53	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037851	M	M	NA	Agree	0.00	5.96	0.00	28.49	12.69	56.18	NONE	POLYA	0.835498	0.105651	0.055528	0.003323	80.06	1.86	17.87	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037852	M	M	NA	Agree	0.00	5.01	0.00	23.96	11.90	59.80	NONE	POLYA	0.858786	0.08946	0.048372	0.003382	80.19	1.82	17.78	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037853	M	M	NA	Agree	0.04	6.08	0.00	28.66	15.36	64.35	NONE	POLYA	0.843561	0.100928	0.052007	0.003505	82.49	1.91	15.40	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6037854	M	M	NA	Agree	0.03	5.51	0.00	24.34	13.78	57.45	NONE	POLYA	0.832651	0.097737	0.048223	0.021389	80.15	3.53	16.10	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does