Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-16) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5964699	M	M	NA	Agree	0.04	3.38	0.00	20.12	6.41	42.39	NONE	POLYA	0.916886	0.023891	0.01979	0.039432	89.92	4.98	4.95	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5964700	M	M	NA	Agree	0.01	3.38	0.00	14.23	6.21	38.19	NONE	POLYA	0.905207	0.035708	0.036693	0.022391	90.14	3.98	5.75	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5964701	M	M	NA	Agree	0.00	2.83	0.00	12.36	5.29	32.98	NONE	POLYA	0.944326	0.014697	0.010248	0.03073	91.32	3.90	4.66	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5964702	M	M	NA	Agree	0.14	7.84	0.00	38.75	11.71	54.70	NONE	POLYA	0.938338	0.029777	0.019986	0.011899	91.71	2.20	5.97	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5964703	M	M	NA	Agree	0.37	6.50	0.00	31.56	10.96	48.91	NONE	POLYA	0.905397	0.041281	0.026844	0.026479	89.98	3.87	5.96	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5964704	M	M	NA	Agree	0.00	5.63	0.00	34.47	10.89	55.72	NONE	POLYA	0.904807	0.049353	0.030367	0.015473	90.11	2.92	6.79	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5964705	M	M	NA	Agree	0.00	1.43	0.00	10.25	3.94	27.02	NONE	POLYA	0.91083	0.030797	0.021638	0.036734	88.81	5.52	5.32	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5964706	M	M	NA	Agree	0.05	1.82	0.00	10.47	3.62	23.51	NONE	POLYA	0.910363	0.042002	0.01989	0.027746	91.98	4.43	3.31	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5964707	M	M	NA	Agree	0.00	1.07	0.00	7.65	2.30	18.25	NONE	POLYA	0.914738	0.032527	0.020538	0.032197	90.02	4.83	4.85	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5964708	M	M	NA	Agree	0.02	1.33	0.00	10.09	2.93	15.76	NONE	POLYA	0.94333	0.027599	0.016975	0.012096	93.39	2.72	3.69	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5964709	M	M	NA	Agree	0.01	1.90	0.00	12.89	2.90	23.17	NONE	POLYA	0.929828	0.033489	0.019001	0.017683	93.36	3.27	3.15	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5964710	M	M	NA	Agree	0.03	2.12	0.00	14.46	4.07	19.92	NONE	POLYA	0.917303	0.044267	0.018752	0.019678	93.38	3.19	3.15	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does