Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5957103	M	M	NA	Agree	0.02	5.93	0.00	30.74	13.68	55.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.881249	0.0662	0.049259	0.003292	96.23	1.80	1.82	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957104	M	M	NA	Agree	0.00	5.84	0.00	29.92	12.59	56.73	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864953	0.082263	0.049129	0.003655	96.21	1.78	1.85	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957105	M	M	NA	Agree	0.01	5.32	0.00	30.62	13.31	55.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886842	0.060095	0.048835	0.004228	96.07	1.95	1.82	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957106	M	M	NA	Agree	0.02	6.22	0.00	34.96	13.91	55.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858578	0.089089	0.048892	0.003442	96.36	1.78	1.69	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957107	M	M	NA	Agree	0.01	7.07	0.00	40.10	16.44	64.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.848566	0.102914	0.046075	0.002445	96.50	1.65	1.68	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957108	M	M	NA	Agree	0.02	5.91	0.00	39.21	14.36	73.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.853119	0.099173	0.045247	0.002461	96.74	1.56	1.54	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957109	M	M	NA	Agree	0.01	6.74	0.00	32.82	14.49	71.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.867164	0.082612	0.047799	0.002425	96.41	1.64	1.80	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957110	M	M	NA	Agree	0.03	6.61	0.00	42.99	15.47	72.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.870795	0.081046	0.045953	0.002206	96.87	1.59	1.38	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957111	M	M	NA	Agree	0.03	5.77	0.00	28.05	15.37	60.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.88494	0.064717	0.048244	0.002099	96.67	1.66	1.53	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957112	M	M	NA	Agree	0.03	5.59	0.00	27.62	14.17	62.86	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.879517	0.068941	0.048334	0.003208	96.48	1.78	1.58	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957113	M	M	NA	Agree	0.00	5.46	0.00	22.58	12.92	57.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.887375	0.065417	0.044578	0.002631	96.30	1.80	1.77	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957114	M	M	NA	Agree	0.02	5.29	0.00	20.87	15.37	48.49	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893502	0.058695	0.04423	0.003574	96.13	1.84	1.87	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957115	M	M	NA	Agree	0.04	7.36	0.00	40.84	16.28	70.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.853426	0.097659	0.046963	0.001952	96.81	1.56	1.47	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957116	M	M	NA	Agree	0.00	6.45	0.00	45.51	17.33	74.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.857367	0.093726	0.046974	0.001933	96.74	1.59	1.50	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957117	M	M	NA	Agree	0.01	7.34	0.00	40.40	15.79	66.83	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.852256	0.09971	0.046426	0.001607	96.76	1.54	1.53	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957118	M	M	NA	Agree	0.01	6.20	0.00	33.77	12.68	61.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.866445	0.084764	0.046481	0.00231	92.24	1.64	5.96	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957119	M	M	NA	Agree	0.01	6.09	0.00	44.84	18.34	69.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858936	0.093472	0.045874	0.001718	96.33	1.54	1.95	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957120	M	M	NA	Agree	0.03	6.38	0.00	36.52	13.13	65.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869259	0.083762	0.044926	0.002053	96.52	1.67	1.66	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957121	M	M	NA	Agree	0.01	5.98	0.00	37.42	15.69	63.24	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.857987	0.093533	0.045968	0.002512	96.37	1.61	1.86	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957122	M	M	NA	Agree	0.02	6.35	0.00	41.04	14.93	67.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.854128	0.098825	0.045533	0.001514	96.63	1.50	1.70	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957123	M	M	NA	Agree	0.00	6.77	0.00	44.71	14.75	71.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.849753	0.101306	0.046172	0.002768	96.84	1.64	1.35	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957124	M	M	NA	Agree	0.05	6.43	0.00	38.92	17.12	70.80	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.854051	0.096836	0.047106	0.002007	96.58	1.57	1.68	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957125	M	M	NA	Agree	0.00	8.53	0.00	46.64	16.58	74.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.849318	0.102653	0.045701	0.002328	96.69	1.59	1.53	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5957126	M	M	NA	Agree	0.00	6.57	0.00	36.54	15.38	78.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.872321	0.078728	0.046559	0.002391	96.52	1.59	1.74	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does