Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5820794	F	F	NA	Agree	81.95	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.861269	0.080022	0.048192	0.010518	90.76	2.45	6.60	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5820795	F	F	NA	Agree	90.90	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.86515	0.080771	0.045562	0.008517	91.46	2.19	6.16	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5820796	F	F	NA	Agree	86.13	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.853441	0.084071	0.04568	0.016809	90.47	2.93	6.43	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5820797	F	F	NA	Agree	74.21	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.859377	0.082323	0.045934	0.012366	90.93	2.52	6.37	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5820798	F	F	NA	Agree	92.90	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.855523	0.088053	0.046002	0.010422	90.78	2.36	6.68	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5820799	F	F	NA	Agree	96.68	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.861486	0.083501	0.046912	0.008102	90.44	2.12	7.26	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5820800	F	F	NA	Agree	105.05	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.851837	0.087561	0.046373	0.014228	90.95	2.66	6.20	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5820801	F	F	NA	Agree	75.71	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.86078	0.080326	0.045737	0.013158	91.52	2.62	5.68	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5820802	F	F	NA	Agree	84.79	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.854779	0.08598	0.044443	0.014797	90.85	2.73	6.24	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5820803	F	F	NA	Agree	80.15	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.864198	0.078785	0.045745	0.011272	91.11	2.43	6.29	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5820804	F	F	NA	Agree	70.76	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.856991	0.08308	0.045638	0.014291	91.53	2.69	5.60	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5820805	F	F	NA	Agree	99.49	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.838259	0.098643	0.045816	0.017283	90.30	2.96	6.55	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does