Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5800140	M	M	NA	Agree	0.01	7.66	0.00	41.48	9.82	62.07	NONE	POLYA	0.86546	0.091213	0.027902	0.015425	91.89	3.00	4.91	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5800141	M	M	NA	Agree	0.05	5.48	0.00	37.26	9.86	70.01	NONE	POLYA	0.883188	0.078622	0.028167	0.010022	92.60	2.55	4.67	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5800142	M	M	NA	Agree	0.00	5.62	0.00	35.48	9.20	58.84	NONE	POLYA	0.881664	0.078304	0.029323	0.01071	92.79	2.52	4.51	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5800143	M	M	NA	Agree	0.03	7.40	0.00	41.66	10.92	65.85	NONE	POLYA	0.8708	0.088508	0.029455	0.011237	92.68	2.57	4.56	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5800144	M	M	NA	Agree	0.00	7.60	0.00	42.78	8.73	69.69	NONE	POLYA	0.874963	0.085381	0.02817	0.011487	92.38	2.56	4.88	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5800145	M	M	NA	Agree	0.00	4.24	0.00	33.69	8.15	59.28	NONE	POLYA	0.886036	0.072285	0.026604	0.015075	92.37	2.88	4.57	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5800146	M	M	NA	Agree	0.00	4.57	0.00	36.72	7.47	58.17	NONE	POLYA	0.868325	0.087883	0.029261	0.014532	92.43	2.83	4.55	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5800147	M	M	NA	Agree	0.00	5.81	0.00	39.56	8.18	59.45	NONE	POLYA	0.870003	0.088213	0.02729	0.014494	92.85	2.77	4.20	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5800148	M	M	NA	Agree	0.01	5.43	0.00	37.00	7.95	54.94	NONE	POLYA	0.871114	0.090088	0.027581	0.011217	92.97	2.50	4.36	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5800149	M	M	NA	Agree	0.01	4.80	0.00	27.75	7.73	59.99	NONE	POLYA	0.871855	0.083641	0.032257	0.012247	92.86	2.72	4.26	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5800150	M	M	NA	Agree	0.04	7.00	0.00	42.67	9.45	54.14	NONE	POLYA	0.872566	0.092181	0.029478	0.005775	93.89	1.97	3.98	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5800151	M	M	NA	Agree	0.00	5.83	0.00	39.56	10.96	60.69	NONE	POLYA	0.879016	0.081556	0.028625	0.010803	92.85	2.50	4.46	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does