Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5753613	M	M	NA	Agree	0.01	2.43	0.00	15.30	3.39	15.51	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.876059	0.096242	0.025369	0.00233	83.20	8.32	7.95	264	LP2 (2026-09-30)	PASS	NonPolyA_perM 264 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5753614	M	M	NA	Agree	0.00	2.09	0.00	22.03	2.10	20.34	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89218	0.079873	0.025791	0.002156	82.20	8.40	8.89	120	LP2 (2026-09-30)	PASS	NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5753615	M	M	NA	Agree	0.00	3.16	0.00	27.70	7.73	21.93	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873403	0.093432	0.031585	0.001581	86.37	5.70	7.58	216	LP2 (2026-09-30)	PASS	NonPolyA_perM 216 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5753616	M	M	NA	Agree	0.00	3.19	0.00	29.08	4.36	42.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.880702	0.081102	0.03651	0.001686	86.72	3.84	9.07	143	LP2 (2026-09-30)	PASS	NonPolyA_perM 143 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5753617	M	M	NA	Agree	0.01	1.14	0.00	10.39	2.85	10.92	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.926477	0.050706	0.021443	0.001375	81.58	10.33	7.63	149	LP2 (2026-09-30)	PASS	NonPolyA_perM 149 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5753618	M	M	NA	Agree	0.00	3.16	0.00	32.40	3.46	35.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869004	0.098648	0.029341	0.003008	85.58	6.45	7.50	177	LP2 (2026-09-30)	PASS	NonPolyA_perM 177 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5753619	M	M	NA	Agree	0.04	4.47	0.00	56.21	6.91	48.01	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843813	0.117806	0.035311	0.003071	85.54	4.16	9.83	213	LP2 (2026-09-30)	PASS	NonPolyA_perM 213 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5753620	M	M	NA	Agree	0.01	2.31	0.00	19.01	4.75	16.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890264	0.081015	0.027136	0.001586	82.86	7.44	9.25	223	LP2 (2026-09-30)	PASS	NonPolyA_perM 223 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5753621	M	M	NA	Agree	0.00	1.14	0.00	10.95	1.65	14.29	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925619	0.051224	0.020763	0.002394	80.92	11.69	6.85	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5753622	M	M	NA	Agree	0.05	3.33	0.00	30.74	5.29	29.94	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.84391	0.122387	0.031145	0.002557	86.53	5.77	7.28	295	LP2 (2026-09-30)	PASS	NonPolyA_perM 295 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5753623	M	M	NA	Agree	0.01	3.23	0.00	34.41	5.76	38.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.837855	0.12442	0.034918	0.002808	85.11	5.23	9.17	291	LP2 (2026-09-30)	PASS	NonPolyA_perM 291 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does